- Oncogenic PIK3CA induces centrosome amplification and tolerance to genome doubling.
Oncogenic PIK3CA induces centrosome amplification and tolerance to genome doubling.
Nature communications (2017-11-25)
Inma M Berenjeno, Roberto Piñeiro, Sandra D Castillo, Wayne Pearce, Nicholas McGranahan, Sally M Dewhurst, Valerie Meniel, Nicolai J Birkbak, Evelyn Lau, Laurent Sansregret, Daniele Morelli, Nnennaya Kanu, Shankar Srinivas, Mariona Graupera, Victoria E R Parker, Karen G Montgomery, Larissa S Moniz, Cheryl L Scudamore, Wayne A Phillips, Robert K Semple, Alan Clarke, Charles Swanton, Bart Vanhaesebroeck
PMID29170395
RESUMEN
Mutations in PIK3CA are very frequent in cancer and lead to sustained PI3K pathway activation. The impact of acute expression of mutant PIK3CA during early stages of malignancy is unknown. Using a mouse model to activate the Pik3ca
MATERIALES
Referencia del producto
Marca
Descripción del producto
Sigma-Aldrich
Anticuerpo anti-fosfo-histona H2A.X (Ser139), clon JBW301, clone JBW301, Upstate®, from mouse
Sigma-Aldrich
Anti-β-actina, anticuerpo monoclonal, clone AC-15, purified from hybridoma cell culture
Sigma-Aldrich
Anti-α-tubulina monoclonal antibody produced in mouse, clone B-5-1-2, purified from hybridoma cell culture
Sigma-Aldrich
Tripsina solution from porcine pancreas, 1 ×, sterile-filtered, BioReagent, suitable for cell culture, 2.5 g porcine trypsin per liter in Hanks′ Balanced Salt Solution with phenol red
Sigma-Aldrich
Monoclonal Anti-α-Actinin antibody produced in mouse, clone BM-75.2, ascites fluid