Saltar al contenido
MilliporeSigma

TRIM11 Prevents and Reverses Protein Aggregation and Rescues a Mouse Model of Parkinson's Disease.

Cell reports (2020-12-03)
Guixin Zhu, Dilshan S Harischandra, Shivani Ghaisas, Pengfei Zhang, Wil Prall, Liangqian Huang, Chantal Maghames, Lili Guo, Esteban Luna, Korrie L Mack, Mariana P Torrente, Kelvin C Luk, James Shorter, Xiaolu Yang
RESUMEN

Neurodegenerative diseases are characterized by the formation and propagation of protein aggregates, especially amyloid fibrils. However, what normally suppresses protein misfolding and aggregation in metazoan cells remains incompletely understood. Here, we show that TRIM11, a member of the metazoan tripartite motif (TRIM) family, both prevents the formation of protein aggregates and dissolves pre-existing protein deposits, including amyloid fibrils. These molecular chaperone and disaggregase activities are ATP independent. They enhance folding and solubility of normal proteins and cooperate with TRIM11 SUMO ligase activity to degrade aberrant proteins. TRIM11 abrogates α-synuclein fibrillization and restores viability in cell models of Parkinson's disease (PD). Intracranial adeno-associated viral delivery of TRIM11 mitigates α-synuclein-mediated pathology, neurodegeneration, and motor impairments in a PD mouse model. Other TRIMs can also function as ATP-independent molecular chaperones and disaggregases. Thus, we define TRIMs as a potent and multifunctional protein quality-control system in metazoa, which might be applied to treat neurodegenerative diseases.

MATERIALES
Referencia del producto
Marca
Descripción del producto

Millipore
Nucleasas Benzonase®, ≥250 units/μL, ≥90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Sigma-Aldrich
ANTI-FLAG® M2 monoclonal antibody produced in mouse, 1 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Sigma-Aldrich
Azul de tiazolil Bromuro de tetrazolio, 98%
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Sigma-Aldrich
5′-Trifosfato de adenosina magnesium salt, ≥95%, bacterial
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Sigma-Aldrich
Anticuerpo anti-NeuN, clon A60, clone A60, Chemicon®, from mouse
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Sigma-Aldrich
Proteasa del TEV
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Millipore
Péptido FLAG®, lyophilized powder
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Sigma-Aldrich
Anti-α-tubulina monoclonal antibody produced in mouse, clone DM1A, ascites fluid
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Sigma-Aldrich
Creatine Phosphokinase from rabbit muscle, Type I, salt-free, lyophilized powder, ≥150 units/mg protein
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Sigma-Aldrich
Phosphocreatine di(tris) salt, ≥97% (enzymatic)
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Sigma-Aldrich
Z-Leu-Leu-Leu-al, ≥90% (HPLC)
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Sigma-Aldrich
Citrate Synthase from porcine heart, ammonium sulfate suspension, ≥100 units/mg protein
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Sigma-Aldrich
Anti-Tyrosine Hydroxylase Rabbit pAb, liquid, Calbiochem®
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Roche
Matriz de afinidad anti-HA, from rat IgG1
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Sigma-Aldrich
Anticuerpo anti-TRIM11, from rabbit, purified by affinity chromatography
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Sigma-Aldrich
p-Xylene-bis(N-pyridinium bromide), ≥95% (TLC)
En este momento no podemos mostrarle ni los precios ni la disponibilidad