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G005881

LC/MS/MS Analysis of Drugs of Abuse: Generic Screening on Ascentis® Express F5

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Ascentis Express F5, 10 cm x 2.1 mm I.D., 2.7 μm particles (53569-U)
mobile phase: [A] 5 mM ammonium formate water pH 4.0 formic acid; [B] 5 mM ammonium formate (95:5 acetonitrile:water)
gradient: 20% B for 0.5 min; to % B in 5.5 min; held at 100% B for 3 min
flow rate: 0.3 mL/min
pressure: 2538 psi (175 bar)
column temp.: 35 °C
detector: MS, ESI(+), TIC, m/z 100-1000
injection: 1 μL
sample: each compound 1000 ng/mL in 40:60 (water:methanol)

suitability

application for HPLC

application(s)

forensics and toxicology

Analysis Note

Shown here is a method to rapidly screen a sample for a variety of abused substances using an Ascentis Expres F5 column. The highest quality LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analito

Descripción
    Benzoylecgonine solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®
    Morphine solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®
    Codeine solution, 1 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®
    Oxycodone solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®
    Normeperidine solution, 100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®
    Hydrocodone solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®
    (±)-MDMA solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®
    (±)-Methamphetamine solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®
    Meperidine solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®
    Fentanyl solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®
    (+)-Propoxyphene solution, 1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®
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