G005377

HPLC Analysis of 25-Dihydroxyvitamin D₂, 25-Hydroxyvitamin D₃ and 3-epi-25-Hydroxyvitamin D₃ on Ascentis^® Express F5, 40 °C

application for HPLC

Propiedades

• technique(s): HPLC: suitable
• test parameters: column: Ascentis Express F5, 10 cm x 2.1 mm I.D., 2.7 μm particles (53569-U); mobile phase: [A] 7 mM ammonium formate; [B] methanol (25:75, A:B); flow rate: 0.4 mL/min; pressure: 3553 psi (245 bar); column temp.: 40 °C; detector: UV, 265 nm; ; ESI(+), m/z 100 - 1000; injection: 1 μL; sample: 300 mg/L in 25:75, water:methanol
• suitability: application for HPLC
• application(s): clinical; vitamins, nutraceuticals, and natural products

Descripción

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analito

25-Hydroxyvitamin D2 solution, 50 μg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant^®
25-Hydroxyvitamin D3 solution, 5 μg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant^®
3-epi-25-Hydroxyvitamin D₃, 98% (CP)