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Supelco

Supel™-Select HLB SPE Tube

bed wt. 30 mg, volume 1 mL, pk of 100

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About This Item

UNSPSC Code:
41115712
NACRES:
NB.21

material

PE frit (20 μm)
polypropylene hardware

quality

MS friendly

composition

bed wt., 30 mg

packaging

pk of 100

technique(s)

solid phase extraction (SPE): suitable

surface area

160-420 m2/g

volume

1 mL

matrix

Hydrophilic modified styrene polymer particle platform

matrix active group

polystyrene phase

particle size

50-70 μm

pore size

80-200 Å pore size

application(s)

food and beverages

separation technique

reversed phase

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General description

Retention Mechanism: Reversed-phase
Sample Matrix Compatibility: Aqueous solutions (biological fluids, water)

  • Hydrophilic modified styrene polymer
  • Developed for the extraction of a highly broad range of compounds from aqueous samples
  • Retention predominately based on reversed-phase; however, because the phase is hydrophilic modified, it is amenable to polar compounds as well.
  • Examples of more polar compounds that are retained and recovered include (but not limited to): pyridoxine (logPo/w -0.56), riboflavin (logPo/w -2.02), biotin (logPo/w 0.11).

Application

Supel™-Select HLB SPE Tube may be used as an extraction cartridge for the solid phase extraction and analysis of caffeine and acetaminophen in amniotic fluid using liquid chromatography coupled to tandem mass spectrometry.

Features and Benefits

  • Excellent sample prep performance at a lower price
  • Amenable to generic methodology - save time, money, and headache during method development
  • Greater capacity allows for smaller bed weights = smaller elution volumes = time savings in sample processing
  • Resistance to overdrying allows for more robust methdology
  • Low UV and MS extractables for lower background and greater sensitivity
  • Stringent production and QC guidelines offer greater lot-to-lot, tube-to-tube, and well-to-well consistency for improved accuracy and precision

Legal Information

Supel is a trademark of Sigma-Aldrich Co. LLC

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificados de análisis (COA)

Lot/Batch Number
14287001
13364001
12324901
11388001
10518901

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If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

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LC-MS/MS analysis of acetaminophen and caffeine in amniotic fluid
Burrai.L, et al.
Analytical Methods : Advancing Methods and Applications, 7(2), 405-410 (2015)
Chiedza F Munyeza et al.
Biomedical chromatography : BMC, 30(6), 837-845 (2015-09-18)
Tigecycline (TIG), a derivative of minocycline, is the first in the novel class of glycylcyclines and is currently indicated for the treatment of complicated skin structure and intra-abdominal infections. A selective, accurate and reversed-phase high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS)
Pablo Vazquez-Roig et al.
Analytical and bioanalytical chemistry, 397(7), 2851-2864 (2010-05-01)
A simple and robust method using solid-phase extraction (SPE) and liquid chromatography tandem mass spectrometry (LC-MS/MS) for the simultaneous determination of 14 drugs of abuse and their metabolites (cocainics, amphetamine-like compounds, cannabinoids, and opiates) in surface waters has been developed.
Wei Chen et al.
Chemosphere, 163, 99-107 (2016-08-16)
A sensitive method for simultaneous determination of 20 trace organic chemicals (TOrCs, including preservatives, antioxidants, disinfectants, oestrogens, alkyl-phenols and bisphenol-A) in surface water and wastewater has been developed and validated based on the optimisation of solid-phase extraction (SPE) followed by
Martina Rejtharová et al.
Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment, 34(4), 477-481 (2016-12-06)
Monitoring of steroid esters in blood serum is desirable in order to detect the possible illegal use of natural hormones as growth promoters. A method for the determination of testosterone propionate, testosterone benzoate, testosterone isocaproate, testosterone decanoate and estradiol benzoate

Artículos

Supel-Select HLB SPE for Pharmaceutical Analysis

Select HLB SPE is a hydrophilic polymer phase for analyte extraction from aqueous matrices. Here it’s used for statins in plasma and 48 pharmaceutical analytes in drinking water.

LC/MS/MS Method for Determination of Glyphosate, AMPA, and Glufosinate in Cereals

Glyphosate and related compounds are measured in oatmeal and infant cereal using ion-exchange polymer-based particles for HPLC and SPE. Low level detection was obtained.

Solid Phase Extraction: Reversed-Phase Methodology

Reversed-phase interaction will retain most molecules with hydrophobic character; it is very useful for extracting analytes that are very diverse in structure within the same sample.

Chromatograms

application for SPE, application for LC-MS

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