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ZRB2978

Sigma-Aldrich

Anti-TIM-3/HAVCR2 Antibody, clone 2B7-L1 ZooMAb® Rabbit Monoclonal

newenhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

Sinónimos:

CD366, HAVcr-2, Hepatitis A virus cellular receptor 2, T-cell immunoglobulin and mucin domain-containing protein 3 (TIMD-3), T-cell immunoglobulin mucin receptor 3 (TIM-3), T-cell membrane protein 3

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About This Item

UNSPSC Code:
12352203

biological source

rabbit

Quality Level

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

2B7-L1, recombinant monoclonal

description

recombinant, expressed in HEK 293 cells

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 33.39 kDa
observed mol wt ~52 kDa

purified by

using Protein A

species reactivity

human

packaging

antibody small pack of 25

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Greener Alternative Product

technique(s)

affinity binding assay: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgGκ

epitope sequence

N-terminal half

Protein ID accession no.

UniProt accession no.

greener alternative category

shipped in

ambient

storage temp.

2-8°C

Gene Information

human ... HAVCR2(84868)

General description

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 2B7-L1 is a ZooMAb® recombinant rabbit monoclonal antibody that specifically detects TIM-3/HAVCR2. It targets an epitope within 24 amino acids from the N-terminal half.

Immunogen

KLH-conjugated linear peptide corresponding to 24 amino acids from the N-terminal half of human TIM-3/HAVCR2.

Application

Quality Control Testing

Evaluated by Western Blotting in Daudi cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TIM-3/HAVCR2 in Daudi cell lysate.

Tested Applications

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected TIM-3/HAVCR2 in HepG2 cells.

Affinity Binding Assay: A representative lot of this antibody bound TIM-3/HAVCR2 peptide with at least ten thousand-fold (10,000X) higher than with non-specific control peptide.

Flow Cytometry Analysis: 1 µg from a representative lot detected TIM-3/HAVCR2 in one million human peripheral blood mononuclear cells (PBMC)..

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected TIM-3/HAVCR2 in human tonsil tissue sections and human spleen tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Hepatitis A virus cellular receptor 2 (UniProt: Q8TDQ0; also known as HAVcr-2, T-cell immunoglobulin and mucin domain-containing protein 3 (TIMD-3), T-cell immunoglobulin mucin receptor 3 (TIM-3), T-cell membrane protein 3, CD366 ) is encoded by the HAVCR2 (also known as TIM3, TIMD3) gene (Gene ID: 84868) in human. As a member of the T cell immunoglobulin and mucin domain (TIM) family, TIM-3 is an important protein in the regulation of macrophage activation. TIM-3 is also critical for immune tolerance as it inhibits T-helper type 1 (Th1) mediated auto and alloimmune responses. It is a membrane bound protein and primarily localized on the plasma membrane of immune cells and also localizes to the immunological synapse between CD8+ T-cells and target cells. It is expressed on a variety of immune cells, including; T cells, dendritic cells, natural killer (NK) cells, macrophages, monocytes, and epithelial tissues. Expression is increased on CD4+ and CD8+ T-cells in chronic hepatitis C virus (HCV) infection. TIM-3 plays an important role in T-cell homing in the thymus and lymph and is the receptor for the Galectin-9 an important protein modulating cell-cell and cell matrix interactions. High TIM-3 expression is also associated with many tumors including renal cancer and acute myelogenous leukemia (AML), and its expression is also a target for anti-cancer therapy as anti-TIM-3 antibodies have been shown to eradicate leukemic stem cells (LSCs) in animal models of AML. Targeting Tim-3 kills two birds with one stone by balancing the immune system and eliminating leukemia stem cells (LSCs) in AML. This ZooMAb® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Wang. Z., et al. (2021). Front Immunol. 1:12;618710; Zhao. L., et al. (2019). Scandinavian Journal of Immunology: 10;1111/12825)

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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