ZRB2263

Anti-DHPS Antibody, clone 1K20 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Sinónimos: DHS, DS
• UNSPSC Code: 12352203
• NACRES: NA.43

Propiedades

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1K20, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 41 kDa; observed mol wt ~45 kDa
• purified by: using Protein A
• species reactivity: rat, human
• species reactivity (predicted by homology): horse, canine, porcine, monkey
• packaging: antibody small pack of 25
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• technique(s): affinity binding assay: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: N-terminal half
• Protein ID accession no.: NP_001921
• UniProt accession no.: P49366
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... DHPS(1725)

Descripción

General description

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1K20 is a ZooMAb^® rabbit recombinant monoclonal antibody that detects DHPS. It targets an epitope within 25 amino acids from the N-terminal half.

Immunogen

KLH-conjugated linear peptide corresponding to 25 amino acids from the N-terminal half of Human DHPS.

Application

Quality Control Testing

Evaluated by Western Blotting in Jurkat membrane extract.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected DHPS in Jurkat cell membrane extract.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected His-tagged, full-length, recombinant Human DHPS..

Affinity Binding Assay: A representative lot of this antibody bound DHPS peptide with a KD of 4.0 x 10-8 in an affinity binding assay.

Western Blotting Analysis: A representative lot detected DHPS in Hek293, MIN6, and INS-1 cell lines (Data courtesy of Dr. Raghu Mirmira lab).

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected DHPS in Human small intestine tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Deoxyhypusine synthase (DHPS) (UniProt: P49366, also known as EC:2.5.1.46) is encoded by the DHPS (also known as DS) gene (Gene ID: 1725) in human. Deoxyhypusine synthase (DHPS) is a highly conserved protein. It is involved in the post-translational modification of eukaryotic translation initiation factor 5A (eIF5A). It catalyzes the conversion of a lysine residue on eIF5A to the unique amino acid hypusine, which is essential for the function of eIF5A. Hypusinated eIF5A (eIF5AHyp) controls the translation of mRNAs involved in inflammation. DHPS also plays a crucial role in various cellular processes, including protein synthesis, cell growth, and differentiation, cell proliferation and apoptosis. DHPS is expressed in various tissues and cell types. It is particularly abundant in tissues with high rates of protein synthesis, such as the liver, brain, and testis. Altered levels of DHPS activity have been observed in certain cancers, implicated in neurodegenerative disorders, cardiovascular diseases, and infectious diseases. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Anderson-Baucum, E., et al., (2021) Cell Metab. 33, 1883 93; Ganapathi, M., et al. (2019) Am. J. Hum. Genet. 104, 287-298)

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable