W1754
Agua
PCR Reagent, suitable for PCR
Sinónimos:
H2O
About This Item
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![Agua, calidad para PCR liquid, pkg of 25 mL (03315959001 [1 x 25 ml]), pkg of 25 mL (03315932001 [25 x 1 ml]), pkg of 100 mL (03315843001 [4 x 25 ml])](/deepweb/assets/sigmaaldrich/product/images/326/208/bac83eed-3c88-4738-b4b5-50d3b223dd1c/640/bac83eed-3c88-4738-b4b5-50d3b223dd1c.jpg)
Nombre del producto
Agua, PCR Reagent
grade
PCR Reagent
Quality Level
vapor density
<1 (vs air)
vapor pressure
3 mmHg
sterility
sterile-filtered
form
liquid
packaging
vial of 1.5 mL
technique(s)
PCR: suitable
refractive index
n20/D 1.34 (lit.)
pH
5-7
bp
100 °C (lit.)
mp
0 °C (lit.)
density
1.000 g/mL at 3.98 °C (lit.)
foreign activity
DNase, none detected
RNase, none detected
SMILES string
O
InChI
1S/H2O/h1H2
InChI key
XLYOFNOQVPJJNP-UHFFFAOYSA-N
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Categorías relacionadas
General description
Application
- as a component of the reaction mixture and as a diluting agent in microfluidic RT-qPCR
- as a component of the reaction mixture for the amplification of products from fungal (Trametes versicolor) DNA
- as a diluting agent and as a component of the reaction mixture for the amplification of cDNA
- Water has been used to make up the final volume of the sample in polymerase chain reaction (PCR)
Suitability
Other Notes
Storage Class
12 - Non Combustible Liquids
wgk_germany
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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Method for bacterial genome analysis and detection of pathogens. Minimize false positive PCRs through lab design and reagents tested for use in bacterial PCR applications.
Protocolos
The most common application for qPCR is the measurement of a gene transcript or copy number quantity relative to one or more reference genes using probe detection.
A protocol that can be used as a basic template for qPCR incorporating a detection probe that is specific to a single target. In these reactions, primers and probe are included at a final concentration of 200 nM and are run using LuminoCt® ReadyMix™.
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A protocol that can be used as a basic template for qPCR incorporating up to four detection probes. In these reactions, primers and probe are included at a final concentration of 200 nM and are run using LuminoCt ReadyMix.
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