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V5890

Sigma-Aldrich

VEGF121

recombinant, expressed in HEK 293 cells, suitable for cell culture

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.26

biological source

human

Quality Level

200

recombinant

expressed in HEK 293 cells

description

Vascular Endothelial Growth Factor 121 human

assay

≥95% (SDS-PAGE)

form

lyophilized powder

potency

<10 ng/mL ED50

quality

endotoxin tested

mol wt

monomer 18 kDa (glycosylated)

packaging

pkg of 10 μg
pkg of 50 μg

technique(s)

cell culture | mammalian: suitable

impurities

≤1 EU/μg Endotoxin level

color

white

solubility

water: soluble

UniProt accession no.

P15692

storage temp.

−20°C

Gene Information

human ... VEGFA(7422)

General description

VEGF121 is expressed in human HEK 293 cells as a disulfide linked, glycosylated cytokine with an apparent molecular mass of 37 kDa as a homodimer and 50 kDa as a homotrimer. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.

Biochem/physiol Actions

VEGF121 belongs to the PDGF/VEGF growth factor family characterized by the presence of eight conserved cysteine residues and a cystine knot structure. VEGF is secreted by the majority of tumor cells and initiates angiogenesis by activating endothelial cells of existing blood vessels and promoting their migration.
VEGF has also been implicated in correlation with poor prognosis in breast cancer.
In addition, VEGF is released in rheumatoid arthritis in response to TNF-α, increasing endothelial permeability and stimulating angiogenesis (formation of capillaries).

Analysis Note

The specific activity was determined by the dose-dependent stimulation of the proliferation of HUVEC cells (human umbilical vein endothelial cells).

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Lee M Ellis
Hematology/oncology clinics of North America, 18(5), 1007-1021 (2004-10-12)
This article focuses on the preclinical evidence for activation of the epidermal growth factor receptor (EGFR) in promoting angiogenesis and the efficacy of anti-EGFR agents in inhibiting angiogenesis and tumor growth.
A O Afuwape et al.
Histology and histopathology, 17(3), 961-972 (2002-08-10)
The expansion of the synovial lining of joints in rheumatoid arthritis (RA), and the subsequent invasion by the pannus of underlying cartilage and bone, necessitates an increase in the vascular supply to the synovium, to cope with the increased requirement
Xiaoqiang Tang
Cancer letters, 332(1), 3-10 (2013-01-26)
Breast cancer development largely depends upon the essential contributions from the tumor microenvironment, where several inflammatory cell populations (e.g. macrophages) orchestrate breast cancer development. The majority of tumor-associated macrophages (TAMs) exhibit alternatively activated M2 properties, produce abundant anti-inflammatory factors and
C J Robinson et al.
Journal of cell science, 114(Pt 5), 853-865 (2001-02-22)
Vascular endothelial growth factor (VEGF) is a secreted mitogen highly specific for cultured endothelial cells. In vivo VEGF induces microvascular permeability and plays a central role in both angiogenesis and vasculogenesis. VEGF is a promising target for therapeutic intervention in

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