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T8665

Sigma-Aldrich

3,3′,5,5′-Tetramethylbenzidine

peroxidase substrate, chromogenic, liquid

Sinónimos:

TMB

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.83

Nombre del producto

3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System, peroxidase substrate

Quality Level

300

form

liquid

storage condition

protect from light

shipped in

wet ice

storage temp.

2-8°C

Categorías relacionadas

Application

TMB is a substrate for horseradish peroxidase. Forms a soluble blue end product that may be read at 370 nm or 655 nm. The TMB reaction may be stopped with 0.5 M H2SO4, resulting in a yellow color, and read at 450 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications.
3,3′,5,5′-Tetramethylbenzidine has been used in a study to demonstrate that by measuring substrate absorption values off the absorption maximum, the measuring range of any immunoenzymometric assay can be extended significantly without jeopardizing assay characteristics. 3,3′,5,5′-Tetramethylbenzidine has also been used in a study to understand the formation of CuS concave superstructures with peroxidase-like activity.

Caution

This substrate is very light-sensitive and should be protected from direct sunlight or UV sources.

Physical form

Ready-to-use.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

signalword

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Eye Irrit. 2 - Flam. Liq. 3 - STOT SE 1

target_organs

Eyes,Central nervous system

wgk_germany

WGK 2

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Certificados de análisis (COA)

Lot/Batch Number
SLCJ8626
SLCH1771
SLCD0040
SLCC5061
SLCB5343

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Tomasz Brudek et al.
Molecular neurodegeneration, 12(1), 44-44 (2017-06-09)
Parkinson's' disease (PD) and Multiple System Atrophy (MSA) are progressive brain disorders characterized by intracellular accumulations of α-synuclein and nerve cell loss in specific brain areas. This loss causes problems with movement, balance and/or autonomic functions. Naturally occurring autoantibodies (NAbs)
Sui-Yuan Chang et al.
Scientific reports, 11(1), 8692-8692 (2021-04-24)
A metal nanoparticle composite, namely TPNT1, which contains Au-NP (1 ppm), Ag-NP (5 ppm), ZnO-NP (60 ppm) and ClO2 (42.5 ppm) in aqueous solution was prepared and characterized by spectroscopy, transmission electron microscopy, dynamic light scattering analysis and potentiometric titration. Based on the in
Paweł Kozielewicz et al.
Molecular pharmacology, 97(1), 23-34 (2019-11-11)
Smoothened (SMO) is a GPCR that mediates hedgehog signaling. Hedgehog binds the transmembrane protein Patched, which in turn regulates SMO activation. Overactive SMO signaling is oncogenic and is therefore a clinically established drug target. Here we establish a nanoluciferase bioluminescence
Nico Langer et al.
Forensic science international, 280, 25-34 (2017-09-25)
One of the main reasons for the rise in popularity of synthetic cannabinoids (SCs) is their ability to remain unrecognized in conventional drug screenings. Due to their structural diversity, caused by the constant introduction of new substances to circumvent legal
S Madersbacher et al.
Methods (San Diego, Calif.), 21(1), 41-50 (2000-04-15)
As a glycoprotein hormone, human chorionic gonadotropin (hCG) is not a single molecular entity. This term comprises not only the bioactive heterodimer hCG but also an array of molecular protein backbone and glycosylation variants, such as its free beta (hCGbeta)
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Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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