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T7902

Sigma-Aldrich

Thermolysin from Geobacillus stearothermophilus

powder, BioReagent, 30-350 units/mg protein (E1%/280), suitable for cell culture

Sinónimos:

Protease from Geobacillus stearothermophilus, Thermophilic-bacterial protease

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About This Item

Número de CAS:
9073-78-3
Comisión internacional de enzimas:
3.4.24.27 (BRENDA, IUBMB)
EC Number:
232-973-4
MDL number:
MFCD00165549
UNSPSC Code:
12352204
NACRES:
NA.75

biological source

Geobacillus stearothermophilus

product line

BioReagent

form

powder

specific activity

30-350 units/mg protein (E1%/280)

mol wt

34.6 kDa

purified by

crystallization

technique(s)

cell culture | mammalian: suitable
single cell analysis: suitable

storage temp.

−20°C

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Application

A thermostable (thermophilic) extracellular metalloendopeptidase containing four calcium ions. Cofactors are zinc and calcium. Hydrolyzes protein bonds on the N-terminal side of hydrophobic amino acid residues. The pH optimum is 8.0 and the optimal temperature for activity is 70 °C. Considerably stable from pH 5 to 9.5. Thermolysin has a low cleavage specificity, therefore, it produces a number of short fragments that are suitable for sequencing. Preferential cleavage: X-cleavage-Y-Z where X=any amino acid; Y=Leu, Phe, Ile, Val, Met, Ala and Z is any amino acid other than Pro. Cleavage N-terminal to Leu is preferred over cleavage of N-terminal to Phe which is preferred over the others. Often used to do limited proteolysis for peptide mapping and studies of protein structure and conformational changes.

Unit Definition

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).

Physical form

lyophilized powder containing calcium and sodium acetate buffer salts

Inhibitor

Referencia del producto
Descripción
Precios

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificados de análisis (COA)

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Yuchen Gu et al.
Food chemistry, 141(3), 2682-2690 (2013-07-23)
The importance of soy products in reducing the risk of cardiovascular disease is well documented. Our previous computation study has indicated the presence of several potent ACE inhibitory peptides within soybean proteins which needs to be identified. The aim of
Raymond G Sierra et al.
Acta crystallographica. Section D, Biological crystallography, 68(Pt 11), 1584-1587 (2012-10-24)
An electrospun liquid microjet has been developed that delivers protein microcrystal suspensions at flow rates of 0.14-3.1 µl min(-1) to perform serial femtosecond crystallography (SFX) studies with X-ray lasers. Thermolysin microcrystals flowed at 0.17 µl min(-1) and diffracted to beyond
Evans Menach et al.
Journal of biochemistry, 152(3), 231-239 (2012-06-01)
In the N-terminal domain of thermolysin, two anti-parallel β-strands, Asn112-Ala113-Phe114-Trp115 and Ser118-Gln119-Met120-Val121-Tyr122 are connected by an Asn116-Gly117 turn to form a β-hairpin structure. In this study, we examined the role of Asn116 in the activity and stability of thermolysin by
Simon Cross et al.
Journal of chemical information and modeling, 52(10), 2587-2598 (2012-09-14)
Pharmacophore elucidation approaches are routinely used in drug discovery, primarily with the aim of determining the three-dimensional arrangement of common features shared by ligands interacting at the site of interest; these features can then be used to investigate the structure-activity
Taha A Jan et al.
Cell reports, 36(2), 109358-109358 (2021-07-15)
The utricle is a vestibular sensory organ that requires mechanosensitive hair cells to detect linear acceleration. In neonatal mice, new hair cells are derived from non-sensory supporting cells, yet cell type diversity and mechanisms of cell addition remain poorly characterized.
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