SRP5282

ERK1, active, GST tagged human

PRECISIO^®, recombinant, expressed in E. coli, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Sinónimos: HS44KDAP, HUMKER1A, MAPK3, MGC20180, P44ERK1, P44MAPK, PRKM3
• Número de CAS: 137632-07-6
• UNSPSC Code: 12352200
• NACRES: NA.32

Propiedades

• biological source: human
• recombinant: expressed in E. coli
• product line: PRECISIO^®
• assay: ≥70% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• specific activity: 672-1008 nmol/min·mg
• mol wt: ~72 kDa
• NCBI accession no.: NM_002746
• application(s): cell analysis
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... MAPK3(5595)

Descripción

General description

ERK1 is a protein serine/threonine kinase that is a member of the extracellular signal-regulated kinases (ERKs), also known as mitogen-activated protein kinase 3 (MAPK3) which are activated in response to numerous growth factors and cytokines. ERK1 is ubiquitously distributed in tissues with the highest expression in heart, brain and spinal cord. Activated ERK1 translocates into the nucleus where it phosphorylates various transcription factors (e.g., Elk-1, c-Myc, c-Jun, c-Fos, and C/EBP beta). ERK pathway is necessary for experience-dependent plasticity and for long-term potentiation of synaptic transmission in the developing visual cortex. ERK activation affects the axonal growth by phosphorylation of microtubule-associated proteins and neurofilaments.

Physical form

Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.

Preparation Note

After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable