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Key Documents

SRP4902

Sigma-Aldrich

Adiponectin from mouse

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

Sinónimos:

ACDC, APM-1, Acrp30, AdipoQ, GBP-28

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

biological source

mouse

recombinant

expressed in E. coli

assay

≥90% (SDS-PAGE)

form

lyophilized

mol wt

~30 kDa

packaging

pkg of 25 μg

storage condition

avoid repeated freeze/thaw cycles

impurities

endotoxin, tested

NCBI accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... Adipoq(11450)

General description

Acrp30 (adipocyte complement-related protein), or cytokine adiponectin, is a hormone produced and secreted by differentiated adipocytes. This protein is composed of an N-terminal collagenous domain and a C-terminal globular trimerization domain. It exists in plasma predominantly as trimeric, hexameric or other high-molecular weight forms. The globular subunit gAcrp30 is however absent in plasma and is biologically active. gAcrp30 is the truncated form of full length of Acrp30, and functions as a ligand of AdipoR1.

Application

Adiponectin from mouse has been used for the treatment of 3T3-L1 cells, along with TNFα (tumor necrosis factor), to determine TNFα inhibition of adiponectin activities by examining lipid accumulation and glucose uptake. It has also been used for the pretreatment of TM3 cells, to study its influence on attenuation of proinflammatory cytokine expression in TM3 cells upon treatment with TNF-α or IL-1β (interleukin).

Biochem/physiol Actions

In obese mice and humans, adiponectin mRNA expression is reduced, therefore linking this hormone to energy homeostasis. In incubated mouse muscle and cultured cells, gAcrp30 leads to an increase in fatty acid oxidation. This hormone is also linked to anti-atherogenesis, and in apoE-deficient mice, overexpression of gAcrp30 results in significant attenuation of atherosclerosis. Long-term administration of this hormone in mice results in weight loss without reduction in food intake. This hormone is capable of reducing proliferation of MDA-ERα7 cell line. Studies show that ratio of gAcrp30 to leptin and the ERα(estrogen receptor alpha) status of cells are key in determining BRCA (breast cancer) cell growth.

Physical form

Lyophilized from a 0.2 μm filtered solution in 25 mM Tris, 0.15 M NaCl, pH 7.4 containing 50 μg of bovine serum albumin (BSA) per 1 μg of adiponectin.

Reconstitution

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.
Recontitute in PBS containing at least 0.1% BSA to a concentration of 0.1-1.0 mg/mL. This stock solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

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Interplay of pro- and anti-inflammatory cytokines to determine lipid accretion in adipocytes.
Wang Y, et al.
International Journal of Obesity, 37(11), 1490-1498 (2013)
ACRP30/adiponectin: an adipokine regulating glucose and lipid metabolism.
Berg AH, et al.
Trends in Endocrinology and Metabolism, 13(2), 84-89 (2002)
Dongying Xuan et al.
Journal of cellular physiology, 231(5), 1090-1096 (2015-09-25)
Emerging evidence suggests an important role for epigenetic mechanisms in modulating signals during macrophage polarization and inflammation. JMJD3, a JmjC family histone demethylase necessary for M2 polarization is also required for effective induction of multiple M1 genes by lipopolysaccharide (LPS).
Adiponectin protects Leydig cells against proinflammatory cytokines by suppressing the nuclear factor-?B signaling pathway.
Wu L, et al.
FEBS Journal, 280(16), 3920-3927 (2013)
Balance of adiponectin and leptin modulates breast cancer cell growth.
Grossmann ME et al
Cell Research, 18(11), 1154-1156 (2008)

Artículos

Lipid Induced Insulin Resistance

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