SRP4872

GDF9 human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

• Sinónimos: GDF9, Growth/differentiation factor 9
• UNSPSC Code: 12352202
• NACRES: NA.32

Propiedades

• biological source: human
• recombinant: expressed in E. coli
• assay: ≥98% (HPLC); ≥98% (SDS-PAGE)
• form: lyophilized
• mol wt: ~31.2 kDa
• packaging: pkg of 20 μg
• impurities: endotoxin, tested
• NCBI accession no.: O60383
• UniProt accession no.: O60383
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... GDF9(2661)

Descripción

General description

GDF-9 is a member of the TGF-β superfamily of growth and differentiation factors, and is highly homologous to GDF-3. Unlike most TGF-family members, GDF-9 and GDF-3 are not disulfide-linked dimers. GDF-3 is expressed in adult bone marrow, spleen, thymus, and adipose tissue. GDF-9 is expressed in oocytes and is required for normal ovarian folliculogenesis. Recombinant human GDF-9 is a 31.0 kDa non-disulfide-linked homodimer containing two 135 amino acid polypeptide chains with 6XHis on its N-terminus.

Application

GDF9 has been as a supplement in the basal medium for the maturation of cumulus–oocyte complexes (COCs).

Biochem/physiol Actions

Growth differentiation factor 9 (GDF9) is known to be localized in the cytoplasm. The gene encoding it is localized on human chromosome 5q31.1. It is known to be associated with granulosa cell proliferation and steroid production. Downregulation of the gene is observed in polycystic ovary syndrome.

Physical form

Sterile filtered and lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Reconstitute in water to a concentration of 0.1-1.0 mg/mL. This solution can then be diluted into other aqueous buffers.

Información de seguridad

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable