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Key Documents

SRP3284

Sigma-Aldrich

GASP-1 human

recombinant, expressed in CHO cells, ≥95% (SDS-PAGE), ≥95% (HPLC)

Sinónimos:

GDF-associated serum protein-1

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human

recombinant

expressed in CHO cells

assay

≥95% (HPLC)
≥95% (SDS-PAGE)

form

lyophilized

mol wt

55.0-66.0 kDa

packaging

pkg of 25 μg

concentration

0.0025-0.0040 μg/mL

impurities

<0.1 EU/μg endotoxin, tested

color

white to off-white

UniProt accession no.

Q8TEU8

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... WFIKKN2(124857)

General description

Growth and differentiation factor-associated serum protein-1 (GASP-1) is expressed primarily in the ovary, testis, and brain. The GASP-1 gene encodes a 571 amino acid protein that contains a 29 amino acid secretion signal sequence, and multiple identifiable structural features, including a WAP domain, a follistatin/Kazal domain, an immunoglobulin domain, two tandem Kunitz domains, and a netrin domain. Recombinant human GASP-1 is a 542 amino acid protein that migrates at an apparent molecular weight of approximately 55-66kDa by SDS-PAGE analysis under non-reducing conditions.

Biochem/physiol Actions

Growth and differentiation factor-associated serum protein-1 (GASP-1) is a secreted inhibitory TGF-β binding protein that contains multiple protease inhibitor structural domains. It can act as a potent soluble inhibitor of myostatin and GDF-11, but not Activin-A.

Physical form

Lyophilized from 10 mM Sodium Phosphate, pH 7.8.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

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Extracellular Regulation of Myostatin: A Molecular Rheostat for Muscle Mass
Se-Jin Lee
Immunology, Endocrine & Metabolic Agents in Medicinal Chemistry, 10, 183-194 (2011)
GASP/WFIKKN Proteins: Evolutionary Aspects of Their Functions
Olivier Monestier
PLoS ONE, 7(8), e43710-e43710 (2012)
Jennifer J Hill et al.
Molecular endocrinology (Baltimore, Md.), 17(6), 1144-1154 (2003-02-22)
Myostatin, a member of the TGFbeta superfamily, is a potent and specific negative regulator of skeletal muscle mass. In serum, myostatin circulates as part of a latent complex containing myostatin propeptide and/or follistatin-related gene (FLRG). Here, we report the identification

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