SRP0280

UbcH13 (UBE2N) Active human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

• Sinónimos: UBC13 homolog, UBE2N, UbcH13, Ubiquitin-conjugating enzyme E2N
• UNSPSC Code: 12352200
• NACRES: NA.32

Propiedades

• biological source: human
• recombinant: expressed in E. coli
• assay: ≥80% (SDS-PAGE)
• form: aqueous solution
• mol wt: 17.6 kDa
• packaging: pkg of 100 μg
• storage condition: avoid repeated freeze/thaw cycles
• concentration: >0.02 mg/mL
• NCBI accession no.: NM_003348
• UniProt accession no.: P61088
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... UBE2N(7334)

Descripción

General description

UbcH13 (GenBank Accession No. NM_003348), amino acids 2-end, with N-terminal His-tag, MW = 17.6 kDa, expressed in an E. coli cell expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.