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Key Documents

SRP0108

Sigma-Aldrich

HDAC-6 human

recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

Sinónimos:

HD6, Histone deacetylase 6, JM21

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human

recombinant

expressed in baculovirus infected insect cells

assay

≥80% (SDS-PAGE)

form

aqueous solution

mol wt

159 kDa

packaging

pkg of 50 μg

NCBI accession no.

BC069243

UniProt accession no.

Q9UBN7

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... HDAC6(10013)

General description

HDAC6 (histone deacetylase 6) is a member of the HDAC family of enzyme which are generally linked with gene expression and chromatin dynamics. This protein resides exclusively in the cytoplasm and is present in the perinuclear punctate structures as well as the leading edges of the cell.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Biochem/physiol Actions

HDAC6 (histone deacetylase 6) functions as a microtubule or tubulin deacetylase, which in turn regulates microtubule-dependent chemotactic cell motility. This protein shows colocalization with p150glued protein, a dynactin component. It contains a ubiquitin binding zinc finger, and interacts with ubiquitinated proteins post-proteasomal inhibition. It is essential for the survival of inflammatory breast cancers (IBCs), though does not function as an oncogene. Treatment of IBC cells with HDAC6 inhibitor ricolinostat (ACY1215) results in the inhibition of IBC cell proliferation both in vitro and in vivo.

Unit Definition

One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37°C.

Physical form

Formulated in 40 mM Tris-HCl pH8.0, 110mM NaCl, 2.2 mM KCl, 0.04% Tween20, 3mM DTT, and 20% glycerol.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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The deacetylase HDAC6 regulates aggresome formation and cell viability in response to misfolded protein stress.
Kawaguchi Y et al
Cell, 115(6), 727-738 (2003)
Charlotte Hubbert et al.
Nature, 417(6887), 455-458 (2002-05-25)
Reversible acetylation of alpha-tubulin has been implicated in regulating microtubule stability and function. The distribution of acetylated alpha-tubulin is tightly controlled and stereotypic. Acetylated alpha-tubulin is most abundant in stable microtubules but is absent from dynamic cellular structures such as
HDAC6 activity is a non-oncogene addiction hub for inflammatory breast cancers.
Putcha P
Breast Cancer Research, 17(1), 149-149 (2015)

Artículos

Histone Modification and Chromatin Remodeling | Epigenetics

Epigenetic modifications are thought to occur through two key interconnected processes—DNA methylation and the covalent modification of histones.

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