SAB4200676

Monoclonal Anti-SPAG11 antibody produced in mouse

clone 10B4, purified from hybridoma cell culture

• Sinónimos: Anti-Spag11, Anti-Spag11a/b, Bin1b, sperm associated antigen 11A/B
• UNSPSC Code: 51111800
• NACRES: NA.41

Propiedades

• biological source: mouse
• Quality Level: 200
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 10B4, monoclonal
• form: buffered aqueous solution
• mol wt: monomer ~7 kDa; ~34 kDa (multimer)
• species reactivity: rat, mouse
• concentration: ~1 mg/mL
• technique(s): immunoblotting: 4-8 μg/mL using whole extracts of HEK-293 cells overexpressing rat SPAG11; immunohistochemistry: 5-10 μg/mL using paraffin-embedded rat caput epididimys sections
• isotype: IgG1
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: mouse ... Spag11a(78128) ; rat ... Spag11a(246305)

Descripción

General description

Monoclonal Anti-SPAG11 (mouse IgG1 isotype) is derived from the hybridoma 10B4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. SPAG11 is expressed specifically in epithelial cells in the middle part of the caput region. It exists in four isoforms such as A, D, E and G.

SPAG11A (sperm associated antigen 11A) belongs to the β defensin family. The protein is mainly expressed in the epididymis. The gene is mapped to human chromosome 8.

Specificity

Monoclonal Anti-SPAG11 recognizes mouse and rat SPAG11.

Immunogen

a recombinant rat SPAG11

Application

Monoclonal Anti-SPAG11 antibody produced in mouse may be used in immunoblotting and immunohistochemistry.

Biochem/physiol Actions

SPAG11 is involved in promoting sperm motility due to its capability to bind the sperm head in different regions of the epididymis. It exhibits anti-microbial activity by suppressing colony growth of Escherichia coli and other Gram-negative bacteria.

SPAG11A (sperm associated antigen 11A) is a secretory protein with possible involvement in sperm maturation. It also controls inflammatory responses in the epididymis.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing or storage in “frost-free” freezer is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable