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S9754

Sigma-Aldrich

Sulfatase from abalone entrails

Type VIII, lyophilized powder, 20-40 units/mg solid

Sinónimos:

Aryl-sulfatase, Aryl-sulfate sulfohydrolase, Phenolsulfatase

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About This Item

Número de CAS:
9016-17-5
Comisión internacional de enzimas:
3.1.6.1 (BRENDA, IUBMB)
EC Number:
232-772-1
MDL number:
MFCD00132399
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

abalone entrails

Quality Level

200

type

Type VIII

form

lyophilized powder

specific activity

20-40 units/mg solid

mol wt

~43 kDa by gel filtration

foreign activity

β-Glucuronidase ≤10.0 Sigma units/mg solid

storage temp.

−20°C

Categorías relacionadas

Application

Sulfatase from abalone entrails has been used in a study to determine that human p-selectin glycoprotein ligand-1 interacts with the skin-associated chemokine CCL27 via sulfated tyrosines at the PSGL-1 amino terminus. Sulfatase from abalone entrails has also been used in a study to investigate HPLC of sulfate and glutathione conjugates from hamster embryo fibroblasts.
Sulfatase from abalone entrails has been used to digest the conjugated forms into free forms for the analysis of tissue green tea polyphenols (GTPs). It has also been used for the detection of free, glucuronidated or total form of GTPs.

Biochem/physiol Actions

Sulfatases hydrolyze the sulfate esters of complex macromolecules, like glycosaminoglycans and sulfatides. It helps to modulate the sulfation states that determine the action of several physiological molecules.

Unit Definition

One unit will hydrolyze 1.0 μmole of p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C (30 min assay).

substrate

Referencia del producto
Descripción
Precios

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Green Tea Polyphenols and Metabolites in Prostatectomy Tissue: Implications for Cancer Prevention
Wang P, et al.
Cancer Prevention Research (Philadelphia, Pa.), 3(8), 985-993 (2010)
Use of a Phage Display Antibody to Measure the Enzymatic Activity of the Sulfs
Uchimura K, et al.
Test, 480, 51-64 (2010)
Sulfatases: structure, mechanism, biological activity, inhibition, and synthetic utility
Hanson SR, et al.
Angewandte Chemie (International ed. in English), 43(43), 5736-5763 (2004)
B A Merrick et al.
Carcinogenesis, 6(9), 1303-1307 (1985-09-01)
Chromatography of benzo[a]pyrene (BaP) sulfate, glucuronide and glutathione (GSH) conjugate standards were examined by h.p.l.c. on a C8 column as modified by various organic acids and solvents. Sulfate and glucuronide standards were positional isomers derived from BaP-1,3,6,7,9 phenols and BaP-GSH
Quercetin increased bioavailability and decreased methylation of green tea polyphenols in vitro and in vivo
Wang P, et al.
Food, 3(6), 635-642 (2012)
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