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Key Documents

R9001

Sigma-Aldrich

Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae)

Type X, lyophilized powder

Sinónimos:

Transfer RNA, tRNA

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About This Item

Número de CAS:
MDL number:
UNSPSC Code:
41106305
NACRES:
NA.55

grade

for molecular biology

type

Type X

form

lyophilized powder

storage temp.

−20°C

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General description

Transfer RNA (tRNA) is isolated from baker′s yeast by phenol-chloroform extraction and ethanol precipitation.

Application

Suitable for use as a carrier in nucleic acid purification and precipitation.

Components

tRNA is provided as a lyophilized powder.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Mammalian in vitro splicing assays.
A Mayeda et al.
Methods in molecular biology (Clifton, N.J.), 118, 315-321 (1999-11-05)
Sean P Ryder et al.
Methods in molecular biology (Clifton, N.J.), 488, 99-115 (2008-11-05)
The gel mobility shift assay is routinely used to visualize protein-RNA interactions. Its power resides in the ability to resolve free from bound RNA with high resolution in a gel matrix. We review the quantitative application of this approach to
Christophe Maris et al.
Nucleic acids research, 48(8), 4521-4537 (2020-03-15)
The polypyrimidine tract binding protein (PTB) is a multi-domain protein involved in alternative splicing, mRNA localization, stabilization, polyadenylation and translation initiation from internal ribosome entry sites (IRES). In this latter process, PTB promotes viral translation by interacting extensively with complex
A simplified method for combined immunohistochemistry and in-situ hybridization in fresh-frozen, cryocut mouse brain sections.
Newton, S.S., et al.
Brain Res. Protoc., 9, 214-214 (2002)
Crystal E Fagan et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(24), 9716-9721 (2013-05-01)
After four decades of research aimed at understanding tRNA selection on the ribosome, the mechanism by which ribosomal ambiguity (ram) mutations promote miscoding remains unclear. Here, we present two X-ray crystal structures of the Thermus thermophilus 70S ribosome containing 16S

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