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Key Documents

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R3757

Sigma-Aldrich

Reactive Yellow 3−Agarose

saline suspension

Sinónimos:

C.I.13245

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About This Item

MDL number:
MFCD00165714
UNSPSC Code:
23151817
NACRES:
NA.56

form

saline suspension

extent of labeling

1-5 mg per mL

matrix

cross-linked 4% beaded agarose

capacity

≥500 units/mL binding capacity (citrate synthase)

storage temp.

2-8°C

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Application

Reactive Yellow 3-agarose is used in affinity chromatography, protein chromatography and dye resins. Reactive Yellow 3-agarose has been used in immunohistochemical localization studies to show where tyramine N-(hydroxycinnamoyl)transferase (THT) polypeptides occur in opium poppy. Reactive Yellow-agarose has also been used to purify human cholesteryl ester transfer protein.

Physical form

Suspension in 0.5 M NaCl containing preservative

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

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Purification of hydroxycinnamoyl-CoA:tyramine hydroxycinnamoyltransferase from cell-suspension cultures of <I>Solanum tuberosum</I> L. cv. Datura.
Hohlfeld, H., et al.
Planta, 199(1), 166-168 (1996)
S J Jeong et al.
Infection and immunity, 68(9), 5132-5138 (2000-08-19)
A novel immunostimulating factor (ISTF) of Actinobacillus actinomycetemcomitans ATCC 29522 was isolated and characterized as inducing proliferation of mouse B cells and human peripheral blood mononuclear cells. This factor was isolated from the bacterial culture medium and purified by size
T Vogt et al.
Planta, 203(3), 349-361 (1997-01-01)
Uridine 5'-diphosphoglucose:betanidin 5-O- and 6-O-glucosyltransferases (5-GT and 6-GT; EC 2.4.1) catalyze the regiospecific formation of betanin (betanidin 5-O-beta-glucoside) and gomphrenin I (betanidin 6-O-beta-glucoside), respectively. Both enzymes were purified to near homogeneity from cell-suspension cultures of Dorotheanthus bellidiformis, the 5-GT by
X B Yi et al.
Biochimica et biophysica acta, 1310(3), 334-342 (1996-02-29)
Nucleoside diphosphate kinase (NDPK) participates in multiple cellular functions, yet the molecular mechanisms of its involvement are often unknown, given that there are no specific inhibitors for the enzyme from vertebrates. We developed antibodies against NDPK by immunization of rabbits
J M Green et al.
The Journal of biological chemistry, 266(20), 12971-12975 (1991-07-15)
p-Aminobenzoate, a component of the vitamin folate, is one of seven compounds derived from the aromatic precursor chorismate in Escherichia coli. Historically the gene products of pabA and pabB were assumed to be sufficient for de novo p-aminobenzoate biosynthesis. Recent
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