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MilliporeSigma

PP0540

Sigma-Aldrich

GlycoProfile β-Elimination Kit

Sufficient for 24 samples

Sinónimos:

β-Elimination Glycan Kit, Glycan Removal Kit

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.28

conjugate

(O-linked)

storage temp.

room temp

Application

The GlycoProfile β-Elimination kit contains a novel non-reducing reagent to efficiently and specifically remove O-linked carbohydrates from glycoproteins with minimal protein or glycan destruction. Consequently, additional downstream proteomics and glycomic analyses may be employed.

Quantity

The kit includes the reagents and techware for O-deglycosylation of up to 24 samples (200 uL at 1-10 ug/uL of glycoprotein per sample).

Legal Information

GlycoProfile is a trademark of Sigma-Aldrich Co. LLC

Solo componentes del kit

Referencia del producto
Descripción

  • Sodium hydroxide solution, 5.0 M 60 μL

  • Microcon centrifugal filter unit, YM-10 membrane, NMWCO 10 kDa 24 ea

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS

  • Centrifugal Filter Unit

pictograms

FlameCorrosion

signalword

Danger

Hazard Classifications

Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1A

Storage Class

3 - Flammable liquids

flash_point_f

82.0 °F - closed cup

flash_point_c

27.79 °C - closed cup


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Visite la Librería de documentos

Prediction of glycoprotein secondary structure using ATR-FTIR
Lewis, S.P., et al.
Vibrational Spectroscopy, 69, 21-29 (2013)

Artículos

Our's GlycoProfile Beta-Elimination Kit allows researchers to perform complete glycoproteomics research by preserving both the O-glycans and protein, specifically remove o-glycans, label o-glycans prior to analysis, have confidence in uniformity of procedure. While it is known that O-glycosylation plays a major role in regulatory biology (as evident with studies around O-GlcNac and RNAi knockdowns of glycosyltransferases) the development of methods for the study of O-glycosylation is under represented compared to N-glycosylation. The under representation is primarily due to the difficulty in removing O-glycans while keeping both the protein and glycans intact. In contrast to N-glycosylation, there is no single enzyme capable of complete O-deglycosylation, so chemical methods must be employed.

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

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