P7962
Monoclonal Anti-PSTAIR antibody produced in mouse
clone PSTAIR, ascites fluid
Sinónimos:
Anti-CDC2
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About This Item
Productos recomendados
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
PSTAIR, monoclonal
mol wt
antigen 33-34 kDa
contains
15 mM sodium azide
species reactivity
mouse, Japanese quail, human, amago salmon, eel, starfish, lily, carp, onion, goldfish, monkey, Xenopus, ciliates
technique(s)
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 1:4,000 using a COS-7 cell extract
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... CDC2(983)
mouse ... Cdc2a(12534)
Categorías relacionadas
General description
In every eukaryote examined, CDKs contain an evolutionary conserved 16 amino acid sequence called PSTAIR (EGVPSTAIREISLLKE) which distinguishes them from other protein kinases. The PSTAIR motif is involved in complex formation with cyclins. The availability of monoclonal antibody reacting specifically with the PSTAIR sequence enables the subcellular detection and localization of the various CDKs and examination of substrate interactions, in a variety of organisms.
Monoclonal Anti-PSTAIR (mouse IgG1 isotype) is derived from the PSTAIR hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Cyclin-dependent kinase 1 (CDC2/CDK1) belongs to the Ser/Thr protein kinase family. It is located on human chromosome 10q21.
Immunogen
synthetic 16 amino acid oligopeptide containing the PSTAIR sequence conjugated to BSA.
Application
Monoclonal Anti-PSTAIR antibody produced in mouse has been used in:
- western blot analysis
- enzyme-linked immunosorbent assay (ELISA)
- immunoprecipitation
Mouse monoclonal clone PSTAIR anti-PSTAIR antibody may be used for the localization of cyclin-dependent kinases containing the PSTAIR motif using various immunochemical assays including ELISA, immunoblot and immunoprecipitation.
Biochem/physiol Actions
Cyclin-dependent kinase 1 (CDC2/CDK1) controls various mitotic processes including the reorganization of the cytoskeleton, chromosome segregation, and formation and separation of daughter cells. It is essential for the multiplication of mammalian cell. It participates in tumorigenesis.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Referencia del producto
Descripción
Precios
Storage Class
10 - Combustible liquids
wgk_germany
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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Gui Ying Li et al.
Cell research, 14(2), 169-175 (2004-04-30)
CDKs play key roles in controlling cell cycle progression in all eukaryotes. In plants, multiple CDKs are present, among which the best characterized CDKs are PSTAIRE CDKs. In this study, we carried out Western blot, immunoelectron microscopy and antibody treatment
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
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