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P7937

Sigma-Aldrich

Protein Phosphatase-1 Catalytic Subunit, α-Isoform from rabbit

5,000-15,000 units/mg protein, recombinant, expressed in E. coli, lyophilized powder

Sinónimos:

Protein phosphatase

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About This Item

Comisión internacional de enzimas:
3.1.3.16 (BRENDA, IUBMB)
MDL number:
MFCD01635658
UNSPSC Code:
12352202
NACRES:
NA.32

biological source

rabbit

Quality Level

200

recombinant

expressed in E. coli

assay

≥80% (GE)

form

lyophilized powder

specific activity

5,000-15,000 units/mg protein

mol wt

37.5 kDa

technique(s)

inhibition assay: suitable

UniProt accession no.

P62139

storage temp.

−20°C

Gene Information

rabbit ... PPP1CA(100009298)

General description

Protein phosphatase1 (PP1) is a heterodimeric enzyme with serine/threonine phosphatase activity. It comprises a catalytic subunit and a targeting subunit or a specific protein inhibitor. The targeting subunit determines the enzyme localization, e.g., to glycogen particles (subunit G) or to myofibrils (subunit M).

Application

Protein Phosphatase-1 Catalytic Subunit, α-Isoform from rabbit has been used as a standard in protein phosphatase-1 inhibition assay.
Protein Phosphatase-1 Catalytic Subunit, α-Isoform from rabbit has been used in protein phosphatase reaction and in the digestion of adipocyte extracts.

Biochem/physiol Actions

Protein phosphatase1 (PP1) is involved in glycogen metabolism and in regulation of muscle contractility. In addition, it is implicated in cell cycle and transcriptional regulation. Recombinant rabbit PP1α has properties in common with the native rabbit muscle protein including size, requirement for Mn2+ for activity, specificity for phosphorylase A, and inhibition by okadaic acid, microcystin LR, and phosphatase inhibitor2 (I-2).

Packaging

Package size based on protein content.

Unit Definition

One unit will hydrolyze 1 nmole of p-nitrophenyl phosphate per min at pH 7.4 at 30 °C.

Physical form

Lyophilized powder containing imidazole buffer, pH 7.4, NaCl, DTT, EDTA, MnCl2, Tween 20, and trehalose as stabilizer.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Regulation of protein phosphatase-1
James B Aggen
Chemistry and Biology, 7.1, R13-R23 (2000)
Activity of membrane-associated sucrose synthase is regulated
by its phosphorylation status in cultured cells of sycamore (Acer
pseudoplatanus)
Javier P
Physiologia Plantarum (2004)
Protein kinases and phosphatases: the yin and yang of protein phosphorylation and signaling.
T Hunter
Cell, 80(2), 225-236 (1995-01-27)
M C Faux et al.
Trends in biochemical sciences, 21(8), 312-315 (1996-08-01)
One important regulatory mechanism in the control of phosphorylation events is the subcellular location of phosphatases of kinases. Several serine/threonine phosphatases and kinases have now been shown to be associated with targeting subunits; these contribute to the organization and specificity
Insulin resistance of glycogen synthase mediated by o-linked N-acetylglucosamine.
Parker GJ
The Journal of Biological Chemistry, 278(12), 10022-10027 (2003)
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