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MilliporeSigma

P5412

Sigma-Aldrich

o-Phenylenediamine

peroxidase substrate, chromogenic, tablet

Sinónimos:

1,2-Diaminobenzene, 1,2-Phenylenediamine, OPD

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About This Item

Fórmula empírica (notación de Hill):
C6H8N2
Número de CAS:
Peso molecular:
108.14
Beilstein/REAXYS Number:
606074
EC Number:
MDL number:
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.83

product name

o-Phenylenediamine, tablet, 20 mg substrate per tablet

vapor density

3.7 (vs air)

Quality Level

vapor pressure

0.01 mmHg ( 25 °C)

form

tablet

bp

256-258 °C

mp

100-102 °C

fluorescence

λex 337 nm; λem 417 nm (after derivatization)

storage temp.

2-8°C

SMILES string

Nc1ccccc1N

InChI

1S/C6H8N2/c7-5-3-1-2-4-6(5)8/h1-4H,7-8H2

InChI key

GEYOCULIXLDCMW-UHFFFAOYSA-N

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Application

o-Phenylenediamine Free Base is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.
o-Phenylenediamine has been used to detect transforming growth factor (TGF-β) and to measure anti-cTfRMAb-ScFv fusion protein using enzyme linked immunosorbent assay (ELISA).

Biochem/physiol Actions

o-Phenylenediamine (o-PD) is a potent substrate for spectrophotometric horseradish peroxidase (HRP)-mediated enzyme-linked immunosorbent assay (ELISA), which gives 2,3-diaminophenazine (DAP) as a product of o-PD oxidation.

Reconstitution

Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.

signalword

Danger

Hazard Classifications

Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 3

flash_point_f

276.8 °F - closed cup

flash_point_c

136 °C - closed cup


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Application of o-phenylenediamine as a fluorogenic substrate in peroxidase-mediated enzyme-linked immunosorbent assay
Mekler VM and Bystryak SM
Analytica Chimica Acta, 264(2), 359-363 (1992)
Receptor-mediated abeta amyloid antibody targeting to Alzheimer?s disease mouse brain
Zhou QH, et al.
Molecular Pharmaceutics, 8(1), 280-285 (2010)
Interleukin-13 Regulates Secretion of the Tumor Growth Factor-beta Superfamily Cytokine Activin A in Allergic Airway Inflammation
Hardy CL, et al.
American Journal of Respiratory Cell and Molecular Biology, 42(6), 667-675 (2010)
Jonas R Knudsen et al.
International journal of molecular sciences, 21(7) (2020-04-02)
The small molecule kinase inhibitor SBI-0206965 was originally described as a specific inhibitor of ULK1/2. More recently, it was reported to effectively inhibit AMPK and several studies now report its use as an AMPK inhibitor. Currently, we investigated the specificity
Breno S Diniz et al.
The journals of gerontology. Series B, Psychological sciences and social sciences, 69(6), 845-851 (2014-08-26)
Late-life depression (LLD) is associated with reduced neurotrophic support and abnormalities in neurodegenerative cascades. The aim of the present study is to determine the concentrations of brain-derived neurotrophic factor (BDNF), amyloid-β42, total Tau, and phosphorylated Tau in the cerebrospinal fluid

Artículos

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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