P1247

Anti-Peroxiredoxin 3 antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Sinónimos: Anti-AOP1, Anti-Antioxidant Protein 1, Anti-PRDX3
• MDL number: MFCD06798479
• UNSPSC Code: 12352203
• NACRES: NA.41

Propiedades

• biological source: rabbit
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~25 kDa
• species reactivity: human
• technique(s): microarray: suitable; western blot: 1:4,000-1:8,000 using HeLa cell mitochondria extracts
• UniProt accession no.: P30048
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... PRDX3(10935)

Descripción

General description

PRDX3, also known as antioxidant protein 1 (AOP1), MER-5, or SP-22, belongs to a family of proteins termed peroxiredoxins (Prx). It is a mitochondrial protein.

The PRDX3 gene is located on the human chromosome at 10q26.11.

Specificity

Whole antiserum is purified to provide an IgG fraction of antiserum Anti-Peroxiredoxin 3 recognizes human PRDX3.

Immunogen

synthetic peptide corresponding to amino acids 241-256 of human peroxiredoxin 3, conjugated to KLH via an N-terminal lysine residue.

Application

Anti-Peroxiredoxin 3 antibody produced in rabbit has been used in western blotting.

Biochem/physiol Actions

Peroxiredoxin 3 (PRDX3) catalyzes the reduction of peroxides in the presence of thioredoxin. The Prx family has been identified in association with a variety of diverse cellular functions including growth control, proliferation, differentiation, and in the response to oxidative stress. PRDX3 is required to maintain normal mitochondrial function through direct activation by c-Myc, which may explain its overexpression during neoplastic transformation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in frost-free freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Storage Class: 11 - Combustible Solids