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OGS1367

PSF-CMV-PURO-NH2-KRYFP - N-TERMINAL YFP TAG MAMMALIAN PLASMID

Name: PSF-CMV-PURO-NH2-KRYFP - N-TERMINAL YFP TAG MAMMALIAN PLASMID
Brand: SIGMA

plasmid vector for molecular cloning

Sinónimos:

cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector

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About This Item

UNSPSC Code:

12352200

NACRES:

NA.85

tag

yellow fluorescent protein (YFP) tagged

form

buffered aqueous solution

mol wt

size 6854 bp

bacteria selection

kanamycin

mammalian cells selection

puromycin

origin of replication

pUC (500 copies)

peptide cleavage

no cleavage

peptide tag location

N-terminal

promoter

Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian

reporter gene

YFP

shipped in

ambient

storage temp.

−20°C

General description

This plasmid is designed to express tagged proteins in mammalian cells either by transient transfection or by creating stable cell lines. It contains a puromycin resistance expression cassette using the human Ubiquitin promoter to drive expression and allow for the selection of cells containing the plasmid.

About the Peptide Tag:This plasmid contains an n-terminal non-aequorea based yellow fluorescent protein (KrYFP) reporter tag that can be fused to a gene of interest to allow protein detection.

Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.oxgene.com

Related product

Referencia del producto

Descripción

Precios

Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Protocolos

How to Choose a SnapFast™ Expression Vector

Sigma-Aldrich presents a technical article to help you choose the right SnapFast™ vector to meet your needs.

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