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N3628

Sigma-Aldrich

4-Nitrophenyl α-L-fuco­pyran­oside

≥98% (TLC), powder

Sinónimos:

4-Nitrophenyl alpha-L-fucopyranoside

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About This Item

Fórmula empírica (notación de Hill):
C12H15NO7
Número de CAS:
10231-84-2
Peso molecular:
285.25
Beilstein/REAXYS Number:
89535
EC Number:
233-553-3
MDL number:
MFCD00063697
UNSPSC Code:
12352204
PubChem Substance ID:
24897654
NACRES:
NA.32

product name

4-Nitrophenyl α-L-fuco­pyran­oside, ≥98% (TLC)

Quality Level

200

assay

≥98% (TLC)

form

powder

solubility

acetone: 4 mg/mL, clear, colorless

storage temp.

−20°C

SMILES string

C[C@@H]1O[C@@H](OCc2ccc(cc2)[N+]([O-])=O)[C@@H](O)[C@H](O)[C@@H]1O

InChI

1S/C13H17NO7/c1-7-10(15)11(16)12(17)13(21-7)20-6-8-2-4-9(5-3-8)14(18)19/h2-5,7,10-13,15-17H,6H2,1H3/t7-,10+,11+,12-,13+/m0/s1

InChI key

DCCILTHSDFBSCK-RCGNDRPLSA-N

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Application

4-Nitrophenyl α-L-fucopyranoside (αfTM) has been used as a chemical substrate for α-fucosidase to determine carbohydrate-active enzymes (CAZymes) activity. It has also been used as a nitrophenyl-linked substrate in p-nitrophenyl glycoside-based enzyme assays to determine the enzyme activity in cecal samples

Biochem/physiol Actions

4-Nitrophenyl α-L-fucopyranoside is a chromogenic substrate that is used in kinetic studies of α-fucosidases.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Alex Steimle et al.
STAR protocols, 2(1), 100326-100326 (2021-03-06)
The gut microbiome expresses a multitude of enzymes degrading polysaccharides in dietary plant fibers and in host-secreted mucus. The quantitative detection of these glycan-degrading enzymes in fecal samples is important to elucidate the functional activity of the microbiome in health
Mahesh S Desai et al.
Cell, 167(5), 1339-1353 (2016-11-20)
Despite the accepted health benefits of consuming dietary fiber, little is known about the mechanisms by which fiber deprivation impacts the gut microbiota and alters disease risk. Using a gnotobiotic mouse model, in which animals were colonized with a synthetic
Regioselective synthesis of alpha-L-fucosyl-containing disaccharides by use of alpha-L-fucosidases of various origins.
K Ajisaka et al.
Carbohydrate research, 224, 291-299 (1992-02-07)
M H Tomassi et al.
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas, 43(1), 8-12 (2009-12-23)
The manner by which effects of simultaneous mutations combine to change enzymatic activity is not easily predictable because these effects are not always additive in a linear manner. Hence, the characterization of the effects of simultaneous mutations of amino acid
Karen M Polizzi et al.
Biotechnology progress, 22(4), 961-967 (2006-08-08)
Following diversity generation in combinatorial protein engineering, a significant amount of effort is expended in screening the library for improved variants. Pooling, or combining multiple cells into the same assay well when screening, is a means to increase throughput and
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