Saltar al contenido
MilliporeSigma
Todas las fotos(1)

Documentos clave

Ver Toda la documentación

MTOXRCSUP

Sigma-Aldrich

RPTEC Complete Supplement

sterile-filtered, suitable for cell culture, add to 500 ML of basal medium

Sinónimos:

Basal media supplement for huRPTEC

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Todas las fotos(1)

About This Item

UNSPSC Code:
12352207
NACRES:
NA.81

Quality Level

300

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

storage temp.

−20°C

General description

Sigma has developed a variety of specialized media which are intended to provide optimal performance when utilized with specific cell lines. This product consists of 30 mL of RPTEC complete media supplement which is added to basal media and other components listed below to produce 500 mL of complete media needed for optimal growth, maintenance, and culture of Sigma′s Human Renal Proximal Tubule Epithelial Cells (RPTEC). The final RPTEC complete media was developed specifically for use with Sigma′s human RPTEC control SA7K Clone cells and various knockouts (KO).

Application

RPTEC Complete Supplement has been used in SA7K cell line culture for cell characterization.

Preparation Note

Prepare Complete Medium by supplementing 500 mL of MEMα (M4526) with 6.25 mL of L-Glutamine (G7513), 30 mL of RPTEC Complete Supplement (MTOXRCSUP), 0.3 mL of Gentamicin solution (G1397) and 0.03 mL of Amphotericin B solution (A2942). Complete Medium can be stored at 2–8 °C for up to 1 month. This medium is formulated for use with a 5% CO2 in air atmosphere.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number
SLCD1475
SLCB0224
SLBX0489
SLBR8562V
SLBR1097V

¿No ve la versión correcta?

Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.

Busque por un COA

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Development and application of human renal proximal tubule epithelial cells for assessment of compound toxicity.
Li S, et al.
Current Chemical Genomics and Translational Medicine, 11, 19-19 (2017)
Marianne K Vormann et al.
The AAPS journal, 20(5), 90-90 (2018-08-16)
Proximal tubules in the kidney play a crucial role in reabsorbing and eliminating substrates from the body into the urine, leading to high local concentrations of xenobiotics. This makes the proximal tubule a major target for drug toxicity that needs

Artículos

Create a 3D kidney nephrotoxicity model on the AIM Biotech 3D cell culture chip

AIM Biotech’s easy-to-use modular platform for conducting 3D cell culture into your research. The AIM Biotech organ-on-a-chip enables you to run simple assays or create more complex microphysiological systems. The versatile platform is suitable for a wide variety of cell types and is customizable for your specific needs.

Human Renal Proximal Tubule Epithelial Cells (RPTEC) as an Essential Advanced Model to Express Kidney Drug Transporters and Toxicity

Human renal proximal tubule epithelial cells (RPTECs) are commonly used to predict human renal drug permeability and to investigate drug efflux. We have generated transporter knockout (KO) cell lines using CompoZr® Zinc Finger Nuclease (ZFN) technology in a proprietary renal proximal tubule epithelial cell line.

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico