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MilliporeSigma

MINI26

Sigma-Aldrich

PKH26 Red Fluorescent Cell Linker Mini Kit for General Cell Membrane Labeling

Distributed for Phanos Technologies

Sinónimos:

Red PKH membrane label kit

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.32

packaging

pkg of 1 kit

Quality Level

manufacturer/tradename

Distributed for Phanos Technologies

storage condition

protect from light

technique(s)

flow cytometry: suitable

fluorescence

λex 551 nm; λem 567 nm (PKH26 dye)

application(s)

cell analysis
detection

detection method

fluorometric

shipped in

ambient

storage temp.

room temp

General description

This kit provides enough dye for up to 25 samples of 20 million cells each and enough diluent for up to 5 samples of 20 million cells each, when using a 2 mL final staining volume containing a final concentration of 2 x 10-6 M of PKH26 dye. Users must determine the optimal dye and cell concentrations from their cell type(s) and experimental purposes.

Application

PKH26 red fluorescent cell linker mini kit has been used for:
  • labelling microglial, natural killer cells and preadipocytes
  • tracking of viable transplanted adipose-derived stem cells
  • in vitro proliferation studies of bone marrow stem cells
  • general cell membrane labeling, including for in vitro cell labeling and long term in vivo cell tracking.

Linkage

For additional technical details on PKH and CellVue® Fluorescent Cell Linker Dyes including an extensive bibliography, please visit here.

Legal Information

CellVue is a registered trademark of Phanos Technologies

Solo componentes del kit

Referencia del producto
Descripción

  • Diluent C 10 mL

  • PKH26 Cell Linker in ethanol .1 mL

pictograms

FlameExclamation mark

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

Storage Class

3 - Flammable liquids

wgk_germany

WGK 3

flash_point_f

57.2 °F - closed cup

flash_point_c

14 °C - closed cup


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Visite la Librería de documentos

Combined use of decellularized allogeneic artery conduits with autologous transdifferentiated adipose-derived stem cells for facial nerve regeneration in rats
Sun F, et al.
Biomaterials, 32(32), 8118-8128 (2011)
Imaging of peripheral benzodiazepine receptor expression as biomarkers of detrimental versus beneficial glial responses in mouse models of Alzheimer's and other CNS pathologies
Ji B, et al.
The Journal of Neuroscience, 28(47), 12255-12267 (2008)
Transplanted bone marrow stem cells relocate to infarct penumbra and co-express endogenous proliferative and immature neuronal markers in a mouse model of ischemic cerebral stroke
Zhang XM, et al.
BMC Neuroscience, 11(1), 138-138 (2010)
Maria Giovanna Gandolfi et al.
Nanomaterials (Basel, Switzerland), 10(3) (2020-03-04)
Exosomes derived from mesenchymal stem cells are extracellular vesicles released to facilitate cell communication and function. Recently, polylactic acid (PLA), calcium silicates (CaSi), and dicalcium phosphate dihydrate (DCPD) have been used to produce bioresorbable functional mineral-doped porous scaffolds-through thermally induced
Adipose tissue regeneration in vivo using micronized acellular allogenic dermis as an injectable scaffold
Lee HY, et al.
Aesthetic Plastic Surgery, 38(5), 1001-1010 (2014)

Artículos

PKH dyes are easy to use and achieve stable, uniform, and reproducible fluorescent labeling of live cells. PKH dyes are non-toxic membrane stains which produce high signal to noise ratio.

Lipophilic cell tracking dyes enable cancer biologists to track tumor and immune cell functions both in vitro and in vivo. Read the article to choose a right membrane dye kit for cell tracking and proliferation monitoring.

Optimal staining is a key component for studying tumorigenesis and progression. Learn useful tips and techniques for dye applications, including examples from recent studies.

A video about how you can use fluorescent cell tracking dyes in combination with flow and image cytometry to study interactions and fates of different cell types in vitro and in vivo.

Ver todo

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