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MilliporeSigma

MAK087

Sigma-Aldrich

Acid Phosphatase Activity Fluorometric Assay Kit

sufficient for 500 fluorometric tests

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About This Item

EC Number:
UNSPSC Code:
12352200

usage

sufficient for 500 fluorometric tests

detection method

fluorometric

storage temp.

−20°C

Gene Information

General description

The new Acid Phosphatase Fluorometric Assay Kit, MAK525, is now available! Acid phosphatases (AP) are a family of enzymes that non-specifically catalyze the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate at an optimal pH of 4-7. Changes in the levels and activities of AP occur in many pathophysiological conditions including prostate cancer and bone disease.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the detection of acid phosphatase in serum and other biological samples.

Principle

The AP Activity Fluorometric Assay Kit provides a simple and direct procedure for measuring AP activity in serum and other samples. AP activity is determined by the conversion of the non-fluorescent 4-methylumbelliferyl phosphate disodium salt (MUP) to the fluorescent product 4-methylumbelliferone (4-MU) (λex = 360/λem = 440 nm), proportional to the AP activity present. One unit of AP is the amount of enzyme that will hydrolyze 1.0 μmole of MUP per minute at 25 °C.

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Storage Class

12 - Non Combustible Liquids


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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