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MilliporeSigma

MAK041

Sigma-Aldrich

β-Hydroxybutyrate Assay Kit

sufficient for 100 colorimetric tests

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

relevant disease(s)

endocrinological disorders, diabetes; cancer; gastrointestinal diseases

storage temp.

−20°C

General description

β-Hydroxybutyrate, also known as 3-Hydroxy-butyrate, is a ketone body primarily synthesized in the liver from the oxidation of fatty acids. Ketone body formation, ketogenesis, is elevated when blood glucose levels drop. Moderately elevated blood ketone bodies occur normally during fasting and prolonged exercise. During alcoholic or diabetic ketoacidosis, blood levels of β-hydroxybutyrate can rise to abnormally high levels. β-hydroxybutyrate can be used as a marker for hepatic energy metabolism. β-hydroxybutyrate formation increases with excessive acetyl-CoA, and serves as a source of energy to the brain.

Application

β-Hydroxybutyrate Assay Kit has been used
  • to quantify the level of β-hydroxybutyrate in plasma/serum
  • to study the change in myocardial 18F-FDG uptake upon extended ketogenic diet, by measuring the level of plasma ketone bodies
  • to study the role of β-hydroxybutyrate in the treatment of impairment and neuropathic pain in spinal cord-injured mice

Suitability

Suitable for the measurement β-Hydroxybutyrate levels in biological samples including serum and urine

Principle

In this assay, β-Hydroxybutyrate concentration is determined by a coupled enzyme reaction, which results in a colorimetric (450 nm) product, proportional to the β-Hydroxybutyrate present.

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Precios

pictograms

Corrosion

signalword

Danger

hcodes

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1B

Storage Class

8A - Combustible corrosive hazardous materials


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Skeletal muscle IL-6 and regulation of liver metabolism during high-fat diet and exercise training.
Knudsen J G, et al.
Physiological Reports, 4(9), e12788-e12788 (2016)
Yongsheng Lan et al.
PloS one, 13(10), e0205562-e0205562 (2018-10-12)
Aerobic exercise improves cognitive function and adult hippocampal neurogenesis. However, the effects of aerobic exercise combined with strength exercise on cognitive function and adult hippocampal neurogenesis are still unknown. In this study, we established exercise paradigms in rats to mimic
Chao Huang et al.
Glia, 66(2), 256-278 (2017-10-24)
Direct induction of macrophage ramification has been shown to promote an alternative (M2) polarization, suggesting that the ramified morphology may determine the function of immune cells. The ketone body metabolite β-hydroxybutyrate (BHB) elevated in conditions including fasting and low-carbohydrate ketogenic
Mark Evans et al.
Medicine and science in sports and exercise, 51(12), 2506-2515 (2019-11-16)
Preexercise ingestion of exogenous ketones alters the metabolic response to exercise, but effects on exercise performance have been equivocal. On two occasions in a double-blind, randomized crossover design, eight endurance-trained runners performed 1 h of submaximal exercise at approximately 65%
Tumor metabolism, the ketogenic diet and β-hydroxybutyrate: novel approaches to adjuvant brain tumor therapy
Woolf E C, et al.
Frontiers in Molecular Neuroscience, 9, 122-122 (2016)

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