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MilliporeSigma

I6510

Sigma-Aldrich

Monoclonal Anti-Human IgE antibody produced in mouse

clone GE-1, ascites fluid

Sinónimos:

Monoclonal Anti-Human IgE

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

secondary antibodies

clone

GE-1, monoclonal

contains

15 mM sodium azide

technique(s)

indirect ELISA: 1:5,000

isotype

IgG2b

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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General description

Binds human IgE; does not bind human IgG, IgA or IgM.
Monoclonal Anti-Human IgE (mouse IgG2b isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulin E (IgE) antibodies are tetramers with two light chains (κ or λ) and two ε-heavy connected by numerous intrachain disulfide bonds. IgE exists in two isoforms, one is associated with the B cell receptor and the other being secreted by plasma cell. It has a short life span in plasma when compared to receptor bounded IgE.

Specificity

Monoclonal Anti-Human IgE is immunospecific for human IgE. No cross-reactivity with human IgG, IgM or purified light chains is observed.

Application

To confirm that a chimaeric monoclonal antibody had episolon chain, an elisa was performed using monoclonal anti-human IgE antibody (clone GE1) at a 1:1000 dilution.

Biochem/physiol Actions

Immunoglobulin E (IgE) is implicated in acute allergic reactions and chronic inflammatory allergic diseases, such as allergic asthma and chronic urticaria (CU). Receptor bounded IgE aids antigen uptake and modulates the differentiation and proliferation of B cells. It confers immunity against helminthic parasites. IgE is also involved in facilitating type I hypersensitivity reactions. It regulates mast cell homeostasis and also exhibits immunomodulatory effects.

Physical form

The product is provided as ascites fluid with 0.1% sodium azide as a preservative.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Analysis Note

The antibody is tested for reactivity against myeloma IgE and allergy-elevated IgE.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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X Su et al.
Analytical biochemistry, 273(1), 66-72 (1999-08-24)
This work presents a piezoelectric (Pz) immunosensor for the quantification of total human IgE in serum samples. The anti-human IgE is deposited on the surface of the 10 M Hz AT-cut gold coated crystal resonator by self-assembled technique, and serves
Jessy Elst et al.
Methods in molecular biology (Clifton, N.J.), 2163, 213-218 (2020-08-09)
The basis of traditional flow cytometry allergy diagnosis is measurement of the expression of basophilic surface activation and/or degranulation markers. Basophils, upon encounter with a specific allergen that cross-links surface FcRI-bound IgE antibodies, not only secrete and release quantifiable bioactive
P Demoly et al.
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 32(7), 1071-1076 (2002-07-09)
Cupressaceae pollen allergy is a world-wide pollinosis but immunotherapy has rarely been tested. Immunotherapy is usually allergen-specific but new forms may be targeted towards IgE. A randomized, double-blind, placebo-controlled trial was carried out to assess the efficacy of a vaccine
Hans C Oettgen
The Journal of allergy and clinical immunology, 137(6), 1631-1645 (2016-06-07)
Fifty years ago, after a long search, IgE emerged as the circulating factor responsible for triggering allergic reactions. Its extremely low concentration in plasma created significant hurdles for scientists working to reveal its identity. We now know that IgE levels
Arnau Navinés-Ferrer et al.
Journal of immunology research, 2016, 8163803-8163803 (2017-01-18)
IgE is an immunoglobulin that plays a central role in acute allergic reactions and chronic inflammatory allergic diseases. The development of a drug able to neutralize this antibody represents a breakthrough in the treatment of inflammatory pathologies with a probable

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