I0408
Invertase Glycoprotein Standard
BioReagent, from Saccharomyces cerevisiae, for proteomics
Sinónimos:
Invertase from baker’s yeast (S. cerevisiae), β-D-Fructofuranosidase, β-D-Fructofuranoside fructohydrolase, Saccharase
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About This Item
Productos recomendados
biological source
Saccharomyces cerevisiae
Quality Level
grade
for proteomics
product line
BioReagent
form
lyophilized powder
mol wt
60 kDa
concentration
≥0.5 mg/vial protein (E1%/280)
storage temp.
2-8°C
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Application
Invertase Glycoprotein Standard has been used:
- to generate N-linked glycan library
- as a negative control to study the binding of lectins to high mannose structures
- for sample pre-treatment in proteomic analyses to study drug-induced toxic epidermal necrolysis
The Invertase Glycoprotein Standard can be used to demonstrate N-glycosylation using PNGase F with both in-solution and in-gel procedures. The extent of deglycosylation can be assessed by mobility shift on SDS-PAGE gels.
Used in the production of confectionary foods and artificial honey.
Biochem/physiol Actions
Invertase hydrolyzes sucrose into glucose and fructose yielding a colorless product, unlike acid hydrolysis which produces colored products.
Other Notes
Invertase is an enzyme that catalyses the hydrolysis of sucrose into fructose and glucose. Invertase Glycoprotein Standard is the periplasmic (glycosylated form, external invertase) with 50% of its mass as polymannan. Since yeast can provide an alternative system for protein glycosylation that is similar to mammalian systems, periplasmic invertase is often used as a model for the study of the function of oligosaccharides in glycoproteins and for studies on glycoprotein biosynthesis.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
ppe
Eyeshields, Gloves, type N95 (US)
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