H9950

Hanahan′s Broth (SOB Medium)

Liquid microbial growth medium

• UNSPSC Code: 12352200

Propiedades

• grade: for molecular biology
• sterility: sterile; 0.2 μm filtered
• form: liquid
• suitability: suitable for nonselective for Escherichia coli and Coliforms
• storage temp.: room temp

Descripción

General description

Hanahan′s (SOB) media is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates.

Application

Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.

Features and Benefits

Hanahan′s (SOB) media provides:

• Filter-sterilized, ready-to-use format
• Convenient package size
• Standard formulation

Components

20 g/L Tryptone
5.0 g/L Yeast Extract
10mM NaCl
2.5mM KCl
10mM MgCl₂
10mM MgSO₄

Physical form

20 g/L Tryptone
5.0 g/L Yeast Extract
10mM NaCl
2.5mM KCl
10mM MgCl₂
10mM MgSO₄

Información de seguridad

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable