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MilliporeSigma

H7787

Millipore

HIS-Select® Spin Columns

Sinónimos:

His-Tag Spin Columns

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

shelf life

2 yr at 2‑8 °C

Quality Level

pore size

100 nm pore size

capacity

>0.5 mg/column

storage temp.

2-8°C

General description

Kit contains 3 collection tubes for every spin column.

Application

Pre-packed and ready-to-use HIS-Select Spin Columns allow for fast and consistent purification of small-scale crude cell extracts containing histidine-tagged proteins. The spin columns contain a matrix of silica particles and uses the HIS-Select patented non-charged, hydrophilic nickel chelating linkage chemistry. HIS-Select Spin Columns are highly selective for histidine-tagged proteins and exhibit very low non-specific binding of other proteins. These convenient Spin Columns allow for fast, one-step purification for the isolation of at least 150 μg of histidine-tagged protein in less than 15 minutes.

Features and Benefits

  • High Selectivity for higher purity
  • Convenient and Ready-to-Use
  • Consistent protein binding each use
  • Compatible with CelLytic cell lysis reagents

Legal Information

CelLytic is a trademark of Sigma-Aldrich Co. LLC
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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PloS one, 4(7), e6373-e6373 (2009-07-29)
As the first pathway-specific enzyme in carotenoid biosynthesis, phytoene synthase (PSY) is a prime regulatory target. This includes a number of biotechnological approaches that have successfully increased the carotenoid content in agronomically relevant non-green plant tissues through tissue-specific PSY overexpression.
Tian Luo et al.
Clinical and vaccine immunology : CVI, 17(1), 87-97 (2009-12-04)
Recently, major species-specific antibody epitopes in three immunoreactive tandem repeat proteins (TRPs) of Ehrlichia chaffeensis, TRP32, TRP47, and TRP120, have been identified and molecularly characterized within tandem repeat (TR) regions. In this study, we mapped the major immunodeterminants of the
Michael D BeGora et al.
The Journal of biological chemistry, 285(38), 29147-29155 (2010-07-24)
Three sequential methylations of phosphoethanolamine (PEA) are required for the synthesis of phosphocholine (PCho) in plants. A cDNA encoding an N-methyltransferase that catalyzes the last two methylation steps was cloned from Arabidopsis by heterologous complementation of a Saccharomyces cerevisiae cho2
Ozan Gundogdu et al.
Journal of bacteriology, 193(16), 4238-4249 (2011-06-07)
Campylobacter jejuni is the leading bacterial cause of human gastroenteritis worldwide. Despite stringent microaerobic growth requirements, C. jejuni is ubiquitous in the aerobic environment and so must possess regulatory systems to sense and adapt to external stimuli, such as oxidative
Anne Durand et al.
The Journal of biological chemistry, 281(40), 29558-29567 (2006-07-26)
In Escherichia coli, the ammonia channel AmtB and the P(II) signal transduction protein GlnK constitute an ammonium sensory system that effectively couples the intracellular nitrogen regulation system to external changes in ammonium availability. Binding of GlnK to AmtB apparently inactivates

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