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H1404

Sigma-Aldrich

Hepatocyte Growth Factor human

>95% (SDS-PAGE), recombinant, expressed in Baculovirus infected High-5 cells, lyophilized powder, suitable for cell culture

Sinónimos:

HGF, Hepatopoietin A, Scatter Factor

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About This Item

EC Number:
214-275-1
MDL number:
MFCD00217422
UNSPSC Code:
12352202
NACRES:
NA.77

Nombre del producto

Hepatocyte Growth Factor human, HGF, recombinant, expressed in Baculovirus infected High-5 cells, suitable for cell culture

biological source

human

Quality Level

200

recombinant

expressed in Baculovirus infected High-5 cells

assay

>95% (SDS-PAGE)

form

lyophilized powder

potency

1.0-20.0 ng/mL

quality

endotoxin tested

mol wt

~80 kDa

packaging

pkg of 5x5 μg
pkg of 5 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

<1.0 EU/μg

UniProt accession no.

P14210

storage temp.

−20°C

Gene Information

human ... HGF(3082)

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Categorías relacionadas

Biochem/physiol Actions

Hepatocyte growth factor (HGF) is a potent mitogen for hepatocytes and a variety of cell types including endothelial and epithelial cells, melanocytes, and keratinocytes. It also mediates epithelial morphogenesis. HGF is the ligand for the tyrosine kinase receptor encoded by the met proto-oncogene.

Other Notes

The NSO-expressed recombinant HGF is greater than 95% heterodimeric HGF. The insect cell line-expressed recombinant HGF is a mixture of single chain HGF (predominant) with some heterodimeric HGF. Single chain HGF and heterodimeric HGF are equally active in in vitro assays.

Physical form

Lyophilized from a 0.2 μm filtered solution in 20 mM NaH2PO4 and 500 mM NaCl, pH 7.0 with 50 μg BSA per 1 μg HGF

Preparation Note

Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. After reconstitution, this solution can be stored at 2-8 °C for up to one month or at -20 °C to -70 °C for up to three months. Us a manual defrost freezer and avoid repeated freeze-thaw cycles.

Analysis Note

The biological activity is measured by its ability to induce IL-11 secretion by Saos-2 human osieosarcoma cells.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificados de análisis (COA)

Lot/Batch Number
SLCG7933
SLCF4636
SLCD4440
SLCC0104
SLBZ9529

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MicroRNA (miRNA) plays a pivotal role in cancer biology. Therefore, tumor suppressor (TS) miRNAs are an attractive target for cancer therapy. However, clinical trials have failed due to the difficulties in miRNA delivery, warranting the development of a novel drug
Ryuichi Tatsumi et al.
American journal of physiology. Cell physiology, 297(2), C238-C252 (2009-06-12)
Regenerative coordination and remodeling of the intramuscular motoneuron network and neuromuscular connections are critical for restoring skeletal muscle function and physiological properties. The regulatory mechanisms of such coordination remain unclear, although both attractive and repulsive axon guidance molecules may be
R A Furlong
BioEssays : news and reviews in molecular, cellular and developmental biology, 14(9), 613-617 (1992-09-01)
Hepatocyte growth factor, a potent mitogen for epithelial and other cell types, and scatter factor, a stimulant of epithelial cell motility are identical. In addition to these mitogenic and motogenic functions, the factor has been shown to be an epithelial
Takanori Takebe et al.
Nature protocols, 9(2), 396-409 (2014-01-25)
Generation of functional and vascularized organs from human induced pluripotent stem cells (iPSCs) will facilitate our understanding of human developmental biology and disease modeling, hopefully offering a drug-screening platform and providing novel therapies against end-stage organ failure. Here we describe
Yingjie Liu et al.
Development (Cambridge, England), 136(5), 843-853 (2009-01-30)
Integrin receptors for the extracellular matrix and receptor tyrosine kinase growth factor receptors represent two of the major families of receptors that transduce into cells information about the surrounding environment. Wnt proteins are a major family of signaling molecules that
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Artículos

Hepatocyte Growth Factor (HGF)

Hepatocyte growth factor (HGF) is a potent mitogen for hepatocytes and a variety of other cells, including endothelial and epithelial cells, melanocytes, and keratinocytes.

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