GERPN2235
ECL™ Select Western Blotting Detection Reagent
Cytiva RPN2235
Sinónimos:
chemiluminescent detection reagent, Western blotting detection reagent
About This Item
Productos recomendados
packaging
pkg of 1 kit
manufacturer/tradename
Cytiva RPN2235
General description
Amersham™ ECL™ Select is a high sensitivity reagent for chemiluminescent Western blotting detection. High signal intensity makes Amersham™ ECL™ Select suitable for the most demanding Western blotting applications, allowing for detection of minute protein quantities. Its long lasting signals allow for multiple exposures and a convenient time window between experiment and analysis. Signals can be detected with CCD camera or X-ray film.
Amersham™ ECL™ Select typically exhibits a dynamic range that is complementary to Amersham™ ECL™ Prime, and similar to Amersham™ ECL™ Advance.
Application
Features and Benefits
- Improved sensitivity: Produces a very high signal intensity
- Quantitative measurements: Linear dynamic range allowing for quantitation of medium to very low abundant proteins
- Works with a wide range of antibody dilutions, reducing the need for lengthy optimization
- Optimized for imaging with ImageQuant LAS 4000 (CCD-based imaging) and compatible with Amersham™ Hyperfilm
- Compatible with Rainbow™ Molecular Weight Markers and Amersham™ ECL™ DualVue Western Blotting Markers.
- Optimized for use with Amersham™ Hybond-P (PVDF) membranes and compatible with Amersham™ Hybond® ECL™ (nitrocellulose) membranes
Components
Solution A (Luminol solution, 50 mL)
Solution B (Peroxide solution, 50 mL)
sufficient for 1000 cm2 membrane
complete packsize
for 1000 cm2 membrane
Storage and Stability
Analysis Note
Legal Information
Storage Class
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Artículos
Background and protocols describing the various methods used by molecular biologists to detect samples of protein or nucleic acids bound to membranes.
This page describes troubleshooting strategies in the detection of GST-tagged proteins.
Protocolos
This page describes chemiluminescence detection of GST-tagged proteins with Amersham ECL, Amersham ECL Prime, and Amersham ECL Select from Cytiva.
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