GE28-9893-35
HiLoad® 16/600 Superdex® 200 pg
Cytiva 28-9893-35, column L × I.D. 60 cm × 16 mm, 34 μm avg. part. size
Sinónimos:
16/600 Superdex 200, HiLoad Superdex 200
About This Item
Productos recomendados
material
bed support (polyamide)
glass column
glass fiber (polypropylene reinforced)
mol wt
10000-600000 Da (globular proteins)
packaging
pkg of 1 ea
manufacturer/tradename
Cytiva 28-9893-35
parameter
<1.0 mL/min flow rate
<5 mL sample volume
0.3 Mpa (42 psi)
bed volume
120 mL
column L × I.D.
60 cm × 16 mm
matrix
agarose dextran composite
dextran-agarose particle platform
polymeric
matrix active group
dextran-agarose phase
avg. part. size
34 μm
cleaning
1-14
working range
3-12
separation technique
size exclusion (SEC)
General description
Superdex® prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose.The separation properties of these media are determined by the dextran component. Steep selectivity curves give excellent resolving power for peptides and proteins in the molecular weight ranges, Mr < 10 000 (Superdex® 30 pg), Mr 3 000 to 70 000 (Superdex® 75 pg), and Mr 10 000 600 000 (Superdex® 200 pg). The medium combines high mechanical strength with high hydrophilicity, allowing high flow rates, and minimal nonspecific interactions.
Features and Benefits
- High resolution with short run times and good recovery.
- High capacity at high flow rates.
- Separations can be scaled up to production levels.
- Prepacked for convenience and reproducibility.
- 1/16” fittings for convenient connections to AKTA design systems.
Suitability
Storage and Stability
Analysis Note
Legal Information
signalword
Warning
hcodes
Storage Class
3 - Flammable liquids
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Artículos
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Protocolos
Superdex from Cytiva are SEC media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superdex column.
This page shows how to refold proteins using gel filtration and products from Cytiva.
Samples for chromatographic purification should be clear and free from particulate matter. Simple steps to clarify a sample before beginning purification will avoid clogging the column, can reduce the need for stringent washing procedures, and can extend the life of the chromatographic medium.
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
Contenido relacionado
Superdex® from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a Superdex® column.
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