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Key Documents

GE28-9322-23

Vivaspin® 500, 5 kDa MWCO Polyethersulfone

Cytiva 28-9322-23, pack of 25 pieces

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About This Item

UNSPSC Code:
41104916
NACRES:
NB.24

material

polycarbonate housing
polyethersulfone membrane

sterility

non-sterile

packaging

pack of 25 pieces

manufacturer/tradename

Cytiva 28-9322-23

availability

not available in Japan

tube size

~2.2 mL

working volume

500 μL

pore size

5 kDa MWCO

storage temp.

room temp

General description

Designed for fast, nondenaturing concentration of biological samples by membrane ultrafiltration. Up to 30-fold concentration of the sample can be achieved with recovery of the target molecule typically exceeding 95%.

Vivaspin® sample concentrators are designed for fast, nondenaturing concentration of biological samples by membrane ultrafiltration. Up to 30-fold concentration of the sample can be achieved with recovery of the target molecule typically exceeding 95%. The entire process is performed in a single tube with an upper compartment containing sample and lower compartment separated by a semipermeable membrane with a molecular weight cut-off (MWCO) selected by the user. Centrifugation is applied to force solvent through the membrane, leaving a more concentrated sample in the upper chamber.

Vivaspin® sample concentrators cater for sample volumes from 100 μl to 20 ml, with a range of molecular weight cutoff values from Mr 3 000 to 100 000. For maximum recovery, select a MWCO at least 50% smaller than the molecular size of the species of interest

Features and Benefits

  • Compatible pH range is from pH 1 to 9.
  • Patented dead-stop technology, which ensures that samples cannot be concentrated to dryness and enables direct concentrate recovery
  • One-step sample concentration in a single tube for minimal sample handling and reduced sample loss.
  • Vertical polyethersulfone membrane minimizes membrane blockage and tolerates high flow rates.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Vivaspin is a registered trademark of Sartorius Stedim Biotech GmbH

Storage Class

13 - Non Combustible Solids

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

Troubleshooting Strategies in GST-tagged Protein Purification

This page describes troubleshooting strategies in purification of GST-tagged proteins using Cytiva products.

Protocolos

Western Blotting Sample Preparation

The emphasis of this page is on the rules and good practice in sample preparation for Western Blots.

Desalting/Buffer Exchange and Concentration for Affinity Chromatography of Tagged Proteins

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

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