GE28-9180-08
PD Miditrap G-25
Cytiva 28-9180-08
Sinónimos:
G-25 Miditrap, Miditrap G-25, PD G-25 Miditrap
About This Item
Productos recomendados
feature
wetted part: no
packaging
pkg of 50 ea (columns)
manufacturer/tradename
Cytiva 28-9180-08
storage condition
dry media
bed size
13 mm × 26 mm
bed volume
3.5 mL
column I.D.
13 mm
matrix
cross-linked dextran
particle size
>50 μm (dry)
85-260 μm
cleaning
2-13
working range
2-13
suitability
suitable for bioprocess medium
Categorías relacionadas
General description
PD MidiTrap G-25 is designed for convenient desalting and buffer exchange of biological samples and volumes up to 1.0 mL can be handled. Two alternative application protocols are available, gravity or centrifugation. By using the gravity protocol, a simple cleanup of the sample is done with one or several columns in parallel without any need for a purification system. With the centrifugation protocol, the samples are run in parallel in a standard centrifuge with minimal dilution of the eluted sample. Four adapters are included in each product pack to enable easy centrifugation.
Application
Features and Benefits
- Reliable and reproducible cleanup of proteins and other large biomolecules (>5000 Mr).
- Range of applications such as desalting, buffer exchange, and removal of low-molecular weight compounds.
- High desalting capacity.
- Flexible protocols with ability to use gravity flow or centrifugation.
- Sample preparation in the range of 0.5 to 1.0 mL for gravity and 0.75 to 1.0 mL with centrifugation.
Storage and Stability
signalword
Warning
hcodes
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.
Protocolos
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This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
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