GE17-5279-01
Glutathione Sepharose™ High Performance
Cytiva 17-5279-01, pack of 25 mL
Sinónimos:
Glutathione Sepharose Column
About This Item
Productos recomendados
ligand
glutathione
packaging
pack of 25 mL
manufacturer/tradename
Cytiva 17-5279-01
matrix
highly cross-linked 6% agarose
avg. part. size
34 μm
cleaning
3-12
working range
3-12
capacity
>7 mg/mL binding capacity(medium)
General description
Glutathione Sepharose™ High Performance is used for the purification of GST-tagged proteins from bacterial lysates and other S-transferases or glutathione-dependent proteins. GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves protein antigenicity and function.
Features and Benefits
- 34 μm bead size for improved efficiency and resolution
- Fast, one-step purification of GST-tagged proteins and other glutathione S-trasferase or glutathione-dependent proteins
- Mild conditions during the purification process preserves protein antigenicity and function
Storage and Stability
Analysis Note
Legal Information
signalword
Warning
hcodes
Storage Class
3 - Flammable liquids
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Artículos
This page describes general considerations for purification of GST-tagged proteins using Cytiva products.
The yield of GST-tagged proteins is highly variable, ranging from 1 mg/l to 10 mg/l. The yield depends on various parameters, such as nature of the tagged protein, the host cell, and the culture conditions used.
This page shows troubleshooting strategies for cleavage methods using Cytiva products.
This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from Cytiva. Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B can all be used for cleavage and purification of GST-tagged proteins in batch.
Protocolos
This page how to remove GST tags by enzymatic cleavage using Cytiva products.
This page describes efficient column packing and preparation for affinity chromatography of tagged proteins using Cytiva products.
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
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