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Key Documents

GE17-5123-10

benzamidin Seph 4Fast Flow H-sub

Cytiva 17-5123-10, pack of 25 mL

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About This Item

UNSPSC Code:
13111000
NACRES:
NA.56

ligand

p-aminobenzamidine

packaging

pack of 25 mL

manufacturer/tradename

Cytiva 17-5123-10

storage condition

(0.05 M Sodium Acetate, pH 4.0 in 20% Ethanol)

matrix

highly cross-linked 4% agarose

average diameter

90 μm

cleaning in place

1-9

working range

2-8

storage temp.

2-8°C

Categorías relacionadas

General description

HiTrap® Benzamidine FF (high sub) is an excellent tool for removal of proteolytic activity from a protein.

Application

Benzamidine Sepharose™ 4 Fast FLow (high sub) is an excellent tool for removal and/or purification of trypsin and trypsin-like serine proteases, as well as, zymogens including urokinase and prekallikrein. Removal of serine proteases is easily done directly from serum, monoclonal cell supernatants and bacterial lysates

Features and Benefits

  • Benzamidine Sepharose™ 4 Fast FLow (high sub) is based on highly cross-linked 4% Agarose enabling high fLow rates without losing binding capacity
  • p-aminobenzamidine (pABA) immobilized on Sepharose™ 4 Fast FLow matrix gives high productivity, and allows easy scale-up.
  • Effective removal of thrombin and factor Xa after tag cleavage of recombinant Proteins
  • For convenient, one-step removal and/or purification of trypsin, trypsin-like serine proteases, and zymogens including urokinase and prekallikrein.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Storage Class

3 - Flammable liquids

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

Purification or Removal of Serine Proteases, such as Thrombin and Trypsin, and Zymogens

This page shows how to Purify or remove serine proteases with a Benzamidine Sepharose 4 Fast Flow, Benzamidine Sepharose 4 Fast Flow from Cytiva.

Principles and Standard Conditions for Different Purification Techniques

This page provides information about the principles and standard conditions for different purification techniques for the purification of multiprotein complexes with from Cytiva.

Purifcation or Removal of Serine Proteases, e.g. Thrombin and Trypsin, and Zymogens

This page shows how to remove serine proteases, e.g. thrombin and trypsin, and zymogens from recombinant products using HiTrap Benzamidine FF and Benzamidine Sepharose 4 Fast Flow from Cytiva.

Principles and Standard Conditions for Different Purification Techniques

This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using Cytiva products.

Ver todo

Protocolos

Removal of Thrombin and Factor Xa Using HiTrap® Benzamidine FF (High Sub)

To protect the fusion protein from proteolytic degradation prior to enzymatic cleavage with PreScission Protease, thrombin, or Factor Xa, it may be necessary to remove proteases from the sample. Additionally, following enzymatic cleavage, it may be necessary to remove thrombin or Factor Xa from the sample.

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

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