GE17-0756-04
Glutathione Sepharose™ 4B
Cytiva 17-0756-04, pack of 300 mL
Sinónimos:
Glutathione Column
About This Item
Productos recomendados
ligand
glutathione
packaging
pack of 300 mL
manufacturer/tradename
Cytiva 17-0756-04
matrix
4% agarose
particle size
45-165 μm
average diameter
90 μm
cleaning in place
4-13
working range
4-13
capacity
>25 mg binding capacity (horse liver GST/ml medium)
General description
GST-tagged proteins and other glutathione S-transferases or glutathone-dependent proteins can be purified directly from bacterial lysates using Glutathione Sepharose™ 4B. Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity.
Features and Benefits
- High capacity single step purification of recombinant glutathione S-transferase (GST) tagged proteins
- Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity and functionality.
Storage and Stability
Analysis Note
Legal Information
signalword
Warning
hcodes
Storage Class
3 - Flammable liquids
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Artículos
This page addresses common problems associated with the expression and growth of GST-tagged proteins using Cytiva products.
This page shows how to purify GST-tagged proteins using GST SpinTrap™ from Cytiva.
This page describes general considerations for purification of GST-tagged proteins using Cytiva products.
The yield of GST-tagged proteins is highly variable, ranging from 1 mg/l to 10 mg/l. The yield depends on various parameters, such as nature of the tagged protein, the host cell, and the culture conditions used.
Protocolos
This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from Cytiva.
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