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Key Documents

GE17-0727-20

SOURCE™ 15RPC

Cytiva 17-0727-20, pack of 10 mL

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About This Item

UNSPSC Code:
13111000
NACRES:
NA.56

ligand

matrix surface

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

feature

autoclavable 20 min at 121°C

packaging

pack of 10 mL

manufacturer/tradename

Cytiva 17-0727-20

matrix

polystyrene/divinylbenzene

avg. part. size

15 μm (monosized)

cleaning in place

1-14

working range

1-12

capacity

~10 mg binding capacity(~10 mg BSA /ml medium at 300 cm/h, ~30 mg bacitracin/ml medium at 300 cm/h, ~50 mg insulin/ml medium at 300 cm/h)

suitability

suitable for bioprocess medium

Categorías relacionadas

General description

SOURCE™ 15RPC is designed for reversed phase chromatography (RPC) with full pH range stability and suitable for polishing steps in industrial processes requiring high f low velocities and low back-pressure.

Application

SOURCE™ 15RPC is based on a 15 μm, monosized, rigid polystyrene/divinyl benzene matrix. This gives Low back pressures and high flow rates during operation allowing for high productivity. The 15 μm bead size allows for very high resolution chromatography steps.

High quality assurance standards in combination with the patented production method, particle structure is consistent both bead-to-bead and batch-to-batch.

A controlled pore size distribution and large specific surface area offer excellent capacity for a wide range of molecules, from small peptides and oligonucleotides up to large Proteins. With full pH range stability, SOURCE™ 15RPC is a strong alternative to silica-based RPC matrices.

SOURCE™ 15RPC is available in a range of different bulk pack sizes for easy scale-up and process development. As member of the BioProcess media range, SOURCE™ 15RPC meets industrial demands with security of supply and comprehensive technical and regulatory support.

Features and Benefits

  • Mono-sized 15 μm rigid beads gives Low back pressures and high flow rates allowing for high productivity.
  • Designed for high resolution reversed phase chromatography of Proteins, peptides and oligonucleotides.
  • High chemical stability allowing for wide range of working conditions with good resistance to cleaning conditions at high pH
  • BioProcess medium supported for industrial applications.

Storage and Stability

Store at 4 to 30 °C (20% Ethanol or 70% Acetonitrile)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

SOURCE is a trademark of Cytiva

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Storage Class

3 - Flammable liquids

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

Troubleshooting Reversed Phase Chromatography (RPC)

Poor-quality eluent components can cause a phenomenon referred to as “ghosting”. Trace levels of organic impurities bind to the medium, concentrating during equilibration and sample application. When elution begins, these contaminants appear in the chromatogram as unknown, or “ghost” peaks.

RPC Purification Options and Scale up with SOURCE™ Media

The different stages of working with SOURCE™ (Cytiva) for high-resolution separation are discussed, from purification to polishing and scaling up

Column Packing and Preparation for Hydrophobic Interaction and Reversed Phased Chromatography

This page discusses column packing and preparation techniques for reverse phase chromatography.

HIC as a Capture Step

This page shows Hydrophobic Interaction Chromatography (HIC) in a purification strategy.

Protocolos

Reverse Phased Chromatography (RPC) in Practice

In this section the practical aspect of Reverse Phased Chromatography ( RPC) is discussed including media and column selection and eluent selection and preparation.

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico