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Key Documents

GE17-0573-01

Sephadex® G-50

DNA grade, Cytiva, pack of 25 g

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About This Item

Número de CAS:
9004-54-0
MDL number:
MFCD00132771
UNSPSC Code:
23151817
NACRES:
NA.52

grade

DNA grade

packaging

pack of 25 g

manufacturer/tradename

Cytiva

particle size

50-150 μm (dry)

InChI

1S/C18H32O16/c19-1-5(21)9(23)10(24)6(22)3-31-17-16(30)14(28)12(26)8(34-17)4-32-18-15(29)13(27)11(25)7(2-20)33-18/h1,5-18,20-30H,2-4H2

InChI key

FZWBNHMXJMCXLU-UHFFFAOYSA-N

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General description

Sephadex G-50 grade F is a size exclusion chromatography resin designed for desalting and buffer exchange, and for purification of DNA and oligonucleotides greater than 20 bases in length using spin columns.

Sephadex G-50 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 is a well-established gel filtration resin for desalting and buffer exchange of biomolecules > 30 000 molecular weight, and with a spin protocol can be used for DNA and oligo purification of molecules greater than 20 bases in length.

Features and Benefits

  • For purification of DNA from small molecules by size exclusion.
  • Tested to ensure reproducibly high recovery of DNA.Sephadex™ G-50 DNA Grade is used in illustra™ Autoseq™ G-50 columns, ProbeQuant™ G-50 Micro Columns, MicroSpin™ G-50 Columns, and NICK Columns.
  • Suitable for researchers who prefer to prepare their own columns for purifying nucleic acids.
  • Excellent for rapid purification of newly synthesized oligonucleotides > 10-mers, including oligonucleotide probes, in 100 to 150 µL of deprotection solution using spin-column chromatography.
  • Useful for desalting or buffer exchange of DNA and removal of unincorporated radionucleotides from end-labeled oligonucleotides (at least 10 bases in length) in a volume of 10 to 100 µL.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sephadex is a registered trademark of Cytiva

Storage Class

13 - Non Combustible Solids

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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