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Key Documents

GE17-0485-01

Cytodex® 3 microcarrier beads

Cytiva, pkg of 10 g

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About This Item

Número de CAS:
MDL number:
UNSPSC Code:
41121800
NACRES:
NC.01

material

dextran beads

sterility

non-sterile

packaging

pkg of 10 g

manufacturer/tradename

17-0485-01
Cytiva

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General description

The microcarrier of choice for cells that may be difficult to culture in vitro.

Microcarrier culture is a technique which makes possible the practical high yield culture of anchorage-dependent cells. Cytodex has been specifically developed for the culture of a wide range of animal cells in culture volumes ranging from a few ml to more than 6000 L. Using Cytodex in simple suspension culture systems provides yields of several million cells per mL.

Formed by chemically coupling a thin layer of denatured collagen to the cross-linked dextran matrix, this is the microcarrier of choice for cells that may be difficult to culture in vitro, and particularly for cells with an epithelial like morphology.

Application

Cytodex™3 microcarrier beads are suitable for:
  • the isolation, transportation, and storage of cells
  • cells that might be challenging to culture in vitro, especially cells with an epithelial morphology, sensitive, differentiated, or low in plating efficiency (e.g., endocrine cells, hepatocytes)
  • large-scale production of viruses, cells, and recombinant cell products (e.g.  hormones, nucleic acids, enzymes, interferon)

Features and Benefits

  • Designed for culture of primary cells or those with an epithelioid morphology, especially cells with low plating-efficiency or differentiated or sensitive cell-types which are normally difficult to grow, e.g. hepatocytes, endocrine cells.
  • Contains a layer of acid-denatured porcine collagen (gelatin) on the surface of the microporous matrix.
  • Designed to yield harvested cells of the highest viability.
  • Transparent for easy microscopic examination of attached cells.

Preparation Note

Prior to use, swell beads in PBS (50 mL/g) and autoclave at 120°C.

Legal Information

Cytodex is a registered trademark of Cytiva

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 1


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