G5160
β-Galactosidase from Aspergillus oryzae
≥8.0 units/mg solid
Sinónimos:
β-D-Galactoside galactohydrolase, Lactase from Aspergillus oryzae
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About This Item
Productos recomendados
form
solid
Quality Level
specific activity
≥8.0 units/mg solid
contains
dextrin as extender
storage temp.
−20°C
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General description
β-Galactosidase is a member of the glycoside hydrolase clan A (GH-A) family. It is widely present in microorganisms, which includes both prokaryotes and eukaryotes.
Application
β-Galactosidase from Aspergillus oryzae has been used:
- in in vitro nuclear magnet resonance (NMR)
- as a standard in the β-galactosidase assay to determine the concentration of cell-associated β-galactosidase
- to modify sugar residues on the surface of the mutant slime cells to study the adhesive properties
- for enzymatic hydrolysis of lactose
- for reversed-phase (RP) adsorption
- in the hydrolysis of whey lactose
Biochem/physiol Actions
β-Galactosidase acts on the terminal β-D-galactosyl moieties of disaccharides, glycoconjugates, and polysaccharides. It is useful in mediating transglycosylation reactions such as the synthesis of galactooligosaccharides (GOS). β-Galactosidases might be used for glycobiological and biotechnological applications.
β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.
Unit Definition
1 U hydrolysiert 1.0 μmol o-Nitrophenyl β-D-Galactosid zu o-Nitrophenol und D-Galactose pro Minute beim angegebenen pH- und Temperatur-Wert, falls nicht anders erwähnt.
inhibitor
Referencia del producto
Descripción
Precios
substrate
Referencia del producto
Descripción
Precios
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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