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G2174

Sigma-Aldrich

ββ-Glucuronidasa from limpets (Patella vulgata)

aqueous solution, ≥85,000 units/mL

Sinónimos:

ββ-D-Glucurónido glucuronosohidrolasa

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About This Item

Número de CAS:
9001-45-0
Comisión internacional de enzimas:
3.2.1.31 (BRENDA, IUBMB)
MDL number:
MFCD00130629
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

limpet (Patella vulgata)

Quality Level

200

form

aqueous solution

specific activity

≥85,000 units/mL

secondary activity

≤7,500 units/mL sulfatase

storage temp.

2-8°C

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Application

Nuevo artículo técnico en el que se compara el rendimiento de las diferentes enzimas
Más información sobre los datos de aplicación recientes generados por I+D Sigma para optimizar la hidrólisis de diferentes clases de medicamentos utilizando enzimas de diferentes procedencias y el uso de una enzima purificada mediante cromatografía para reducir el efecto de la actividad esterasa que produce la conversión de 6-MAM en morfina.
Se ha comunicado que la enzima de patella vulgata hidroliza los opioide-glucurónidos con más eficacia que las enzimas de Helix pomatia, de hígado bovino y de E. coli

Features and Benefits

Save time with no reconstitution in this convenient liquid format.

Unit Definition

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 3.8 (30 min assay).
Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.

Physical form

Aqueous solution in 0.9% NaCl with 0.02% sodium azide as preservative.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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J Combie et al.
Clinical chemistry, 28(1), 83-86 (1982-01-01)
beta-Glucuronidase from Patella vulgata, Helix aspersa, Helix pomatia, and bovine liver were evaluated for usefulness in routine hydrolysis of drug-glucuronic acid conjugates from equine urine samples. Factors affecting the reaction rate (enzyme concentration, ligand concentration, temperature, and pH) were optimized.
T A Jennison et al.
Journal of analytical toxicology, 17(4), 208-210 (1993-07-01)
Methods to confirm morphine in urine require hydrolysis to liberate morphine from its 3-beta-D glucuronide (M-3G) conjugate. Lengthy enzyme hydrolysis procedures prolong testing turnaround time whereas rapid enzyme methods may produce a low conversion of M-3G to morphine. The purpose
Victor Aguilar-Hernández et al.
Plant molecular biology, 84(4-5), 429-441 (2013-10-19)
Gene duplication events exert key functions on gene innovations during the evolution of the eukaryotic genomes. A large portion of the total gene content in plants arose from tandem duplications events, which often result in paralog genes with high sequence
Gianluigi Zaza et al.
Experimental biology and medicine (Maywood, N.J.), 239(1), 52-64 (2013-11-06)
Peritoneal (PD) and hemodialysis (HD) represent the leading renal replacement therapies in advanced chronic kidney disease (CKD). Although absolutely necessary to ensure patient survival, these treatments are responsible for considerable biological alterations primarily due to the un-physiological contact of blood
Miribane Dërmaku-Sopjani et al.
Molecular membrane biology, 30(8), 369-385 (2013-10-16)
The Klotho gene was identified as an 'aging suppressor' in mice. Overexpression of the Klotho gene extends lifespan and defective Klotho results in rapid aging and early death. Both the membrane and secreted forms of Klotho have biological activity that
Ver todos los artículos relacionados

Artículos

Evaluation of Different Enzymes on Hydrolysis Efficiencies of Glucuronide Drug Metabolites in Urine

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

Protocolos

LC/MS (TOF) Analysis of Drugs and Their Glucuronide Metabolites in Urine on Titan™ C18 After Solid Phase Extraction (SPE) Using Supel™-Select SCX, β-Glucuronidase Enzyme Digestion

-THC solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material

Using UHPLC/MS (TOF) for Detection of Drugs and Metabolites in Urine Following Optimized Enzymatic Hydrolysis Conditions

To optimize hydrolysis using β-glucuronidase, factors such as incubation time, temperature, hydrolysis pH, enzyme source, and enzyme concentration must be evaluated for each glucuronide metabolite to be analyzed.

Chromatograms

application for SPE, application for HPLC

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