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MilliporeSigma

F4771

Sigma-Aldrich

Monoclonal Anti-Fibroblast Surface Protein antibody produced in mouse

clone 1B10, ascites fluid

Sinónimos:

Fibroblast Surface Protein Antibody, Fibroblast Surface Protein Antibody - Monoclonal Anti-Fibroblast Surface Protein antibody produced in mouse

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

1B10, monoclonal

mol wt

antigen 43-72-80 kDa

contains

15 mM sodium azide

species reactivity

human

technique(s)

complement-mediated cytotoxicity assay: 1:500
flow cytometry: suitable
immunohistochemistry (frozen sections): suitable

isotype

IgM

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Monoclonal Anti-Human Fibroblast Surface Protein (mouse IgM isotype) is derived from the 1B10 hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with cultured human thymic fibroblasts. Human fibroblast surface protein is a fibroblast antigen that is expressed mainly on human synovial, foreskin and thymic fibroblasts and is absent on human epithelial cells and lymphocytes.

Immunogen

cultured human thymic fibroblasts

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)
Monoclonal Anti-Fibroblast Surface Protein antibody produced in mouse has been used in:
  • immunofluorescence
  • flow cytometry
  • immunohistochemistry
  • immunoblotting

Monoclonal anti-fibroblast surface protein antibody can be used in flow cytometry of human fibroblasts and monocytes. It is also useful in immunoblotting and western blotting.

Biochem/physiol Actions

Monoclonal Anti-Fibroblast Surface Protein antibody is also used to remove fibroblasts from human cultured cells by inhibiting fibroblasts adherence to the culture vessel or by cytotoxic effect in the presence of rabbit complement. In addition, apart from the functional significance of cells characteristics, the characterization of cell lines is important for correlation of cultures with tissue origin, identification of the lineage to which the cells belong or the precursor status of the cells. Monoclonal antibodies to fibroblast surface protein should provide a powerful tool for control of fibroblast growth in a variety of human cell culture systems, such as thymic epithelial (TE) cell cultures allowing the growth of highly enriched populations of the TE cells.

Target description

This fibroblast antigen has been demonstrated on human synovial, mammary, foreskin and thymic fibroblasts and in malignant fibrosarcoma tissue. The antigen is both cell membrane and lysosome associated.

Analysis Note

Working dilution is at least 1:500 by complement-mediated microcytotoxicity assay.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Referencia del producto
Descripción
Precios

Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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A Ocklind
Experimental eye research, 67(2), 179-191 (1998-09-12)
Prostaglandin F2alpha and its analogue latanoprost, both prostanoid FP receptor agonists, reduce the intraocular pressure mainly by enhancing uveoscleral outflow. Changes in the extracellular matrix of the ciliary muscle may be involved in the increased outflow. The effect of latanoprost
L Rønnov-Jessen et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 40(4), 475-486 (1992-04-01)
Fibroblasts with smooth muscle differentiation are frequently derived from human breast tissue. Immunofluorescence cytochemistry of a fibroblast-associated antigen recognized by a monoclonal antibody (MAb), 1B10, was analyzed with a view to discriminating smooth muscle differentiated fibroblasts from vascular smooth muscle
Qiyuan Zhou et al.
American journal of respiratory cell and molecular biology, 54(5), 728-739 (2015-10-22)
We have reported that von Hippel-Lindau protein (pVHL) expression is elevated in human and mouse fibrotic lungs and that overexpression of pVHL stimulates fibroblast proliferation. We sought to determine whether loss of pVHL in fibroblasts prevents injury and fibrosis in
Carcinoma-Associated Fibroblast-Like Differentiation of Human Mesenchymal Stem Cells
Mishra PJ, et al.
Cancer research, 68(11), 4331-4339 (2008)
A Dubicke et al.
Molecular human reproduction, 14(11), 641-647 (2008-10-17)
The aims of the present study were to compare the levels of mRNA and protein expression of matrix metalloproteinase (MMP)-1, -3, -8 and -9 in human cervical tissue in preterm and term labor as well as not in labor and

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